| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIFC2 |
| Uniprot No | Q96AC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-838aa |
| 活性数据 | MYAFYSLLIYIFYSLFRRDGGAAAAAEPGDPAQRARKPRGRRRPDLPAPELWTELTGLAASSEPEDGSEGAAEGRAAAVSLEEALLRLAEFLSVQLGAEESCGGPADLGQSGEVPSLLTVTSQLLALLAWLRSPRGRQALLQGTQPAPRVRPPSPDGSTSQEESPSHFTAVPGEPLGDETQGQQPLQLEEDQRAWQRLEQLILGQLEELKQQLEQQEEELGRLRLGVGATDSEKRVQHLTLENEALKQSLSLMRDLLLHWGPGPPIRAPQEEAEALLELQGRLQEAQDTTEALRAQLGVQEVQLQGLQGALQQLQQETEQNCRRELQQMHGQLAGLRARMASLRQGCGDLRGLVSTFTQSCQGSLSEARGQVSWALGALSSGGPGTQLPEGQQGPPAGCPGRLPELKGNIRVLCRLRPGTSSSLVSVEPGPGGTVTTCYRGRHRRFRLDWVFPPDASQEEVFRELEPAVLSCLRGYSVCIFTYGQTGTGKTYSMEGPPEDPGIVPRALQSLFREMGAGRQHRVTLSMVEIYNEAVRDLLAPGPPERLAVRQGPEGQGGIQVAGLTHWDVPNLETLHQMLKLGRSNRATAATAMNQRSSRSHALVTLTLRAASPPRAPGTAGTLHLVDLAGSERARKAGAAGPPRGDPDGARRLREAQTINRSLLALGGVMAALRAHRPHVPFRDSQLTRLLQPALGPGTTAVLLLQVGAGAGQVCACRSPPTRARPPAPLARRSPRGRRISGRQSAPSSSPTEWVKWSWGQPGAAGSRAPPGRLLPSAPTLRSPGPPAPLRRPLAVLHAPVPTTARARLSRPQRACPSSPGSRPCPWGLRPGLCWQRR |
| 分子量 | 90.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIFC2蛋白的虚构参考文献示例(基于领域知识推测性整理，实际文献请查询数据库验证)：
1. **《Molecular cloning and functional characterization of human KIFC2》**
- 作者：Zhang Y, Li X, Wang H
- 摘要：本文首次报道了人源KIFC2基因的克隆及重组蛋白在真核细胞中的表达，发现其通过C端结构域与微管结合，并参与高尔基体囊泡的逆向运输。
2. **《KIFC2 regulates acrosome formation via interaction with spermatid microtubules》**
- 作者：Yang L, Chen R, Yu K
- 摘要：研究利用重组KIFC2蛋白揭示其在精子发生中的功能，证实其通过调控微管动力学参与精子顶体形成，缺失会导致雄性小鼠不育。
3. **《Structural analysis of recombinant KIFC2 reveals ATPase-dependent motility mechanism》**
- 作者：Johnson T, Smith P
- 摘要：通过体外重构实验解析KIFC2的ATP酶活性与运动功能，发现其具有独特的双头结构，活性依赖于微管结合域的构象变化。
4. **《KIFC2 overexpression promotes tumor invasion in human breast cancer cells》**
- 作者：Doe J, Lee S, Kim M
- 摘要：研究利用重组KIFC2蛋白验证其在乳腺癌细胞迁移中的作用，发现其通过稳定细胞质微管网络增强侵袭性，或成为潜在治疗靶点。
(注：以上为模拟内容，实际文献需通过PubMed、Google Scholar等平台以“recombinant human KIFC2”等关键词检索。)
Recombinant human KIFC2 protein is a genetically engineered form of the kinesin superfamily protein C2 (KIFC2), a microtubule-dependent motor protein involved in intracellular transport and mitotic processes. As a member of the kinesin-14 family, KIFC2 features a conserved N-terminal motor domain with ATPase activity that enables microtubule binding and movement, along with a C-terminal tail for cargo interaction. Unlike many kinesins that move toward microtubule plus-ends, KIFC2 exhibits minus-end-directed motility, playing roles in spindle organization, chromosome segregation, and vesicle trafficking. Recombinant KIFC2 is typically produced in heterologous systems like *E. coli* or mammalian cells, allowing researchers to study its structure, biochemical properties, and interactions without interference from native cellular components. Its recombinant form is crucial for *in vitro* assays, structural studies (e.g., cryo-EM), and drug screening, particularly in cancer research, as KIFC2 overexpression has been linked to tumor progression and chemotherapy resistance. Current studies focus on elucidating its regulatory mechanisms in cell division and exploring its therapeutic potential as a target for antimitotic agents. However, its precise physiological roles and disease associations remain under investigation, necessitating further functional and clinical validation.
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