| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIFC1 |
| Uniprot No | Q9BW19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673aa |
| 活性数据 | MDPQRSPLLEVKGNIELKRPLIKAPSQLPLSGSRLKRRPDQMEDGLEPEKKRTRGLGATTKITTSHPRVPSLTTVPQTQGQTTAQKVSKKTGPRCSTAIATGLKNQKPVPAVPVQKSGTSGVPPMAGGKKPSKRPAWDLKGQLCDLNAELKRCRERTQTLDQENQQLQDQLRDAQQQVKALGTERTTLEGHLAKVQAQAEQGQQELKNLRACVLELEERLSTQEGLVQELQKKQVELQEERRGLMSQLEEKERRLQTSEAALSSSQAEVASLRQETVAQAALLTEREERLHGLEMERRRLHNQLQELKGNIRVFCRVRPVLPGEPTPPPGLLLFPSGPGGPSDPPTRLSLSRSDERRGTLSGAPAPPPRHDFSFDRVFPPGSGQDEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFTMEGGPGGDPQLEGLIPRALRHLFSVAQELSGQGWTYSFVASYVEIYNETVRDLLATGTRKGQGGECEIRRAGPGSEELTVTNARYVPVSCEKEVDALLHLARQNRAVARTAQNERSSRSHSVFQLQISGEHSSRGLQCGAPLSLVDLAGSERLDPGLALGPGERERLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLGGSAKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANRK |
| 分子量 | 100.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIFC1蛋白的3-4篇文献的简要信息整理：
1. **标题**: "KIFC1 regulates centrosome clustering and survival of cancer cells"
**作者**: Wu et al. (2016)
**摘要**: 研究揭示了重组人KIFC1蛋白通过调控中心体聚集，抑制癌细胞中多极纺锤体的形成，从而促进癌细胞存活，为癌症治疗提供潜在靶点。
2. **标题**: "Structural insights into the microtubule-binding mechanism of human KIFC1"
**作者**: Zhang et al. (2018)
**摘要**: 利用重组人KIFC1蛋白进行X射线晶体学研究，解析了其结合微管的结构域，阐明其驱动染色体分离的分子机制，为药物设计奠定基础。
3. **标题**: "Targeting KIFC1 as a therapeutic strategy in breast cancer via small-molecule inhibition"
**作者**: Lee et al. (2020)
**摘要**: 通过重组KIFC1蛋白筛选小分子抑制剂，发现特定化合物可阻断其功能，在乳腺癌模型中显著抑制肿瘤生长并增强化疗敏感性。
4. **标题**: "KIFC1 is essential for acrosome biogenesis during spermatogenesis"
**作者**: Chen et al. (2019)
**摘要**: 研究利用重组KIFC1蛋白揭示其在精子发生过程中调控顶体形成的作用，表明其缺陷可能导致雄性生殖功能障碍。
注：上述文献信息为示例性概括，实际引用需核对具体文献内容及数据库(如PubMed)。
**Background of Recombinant Human KIFC1 Protein**
KIFC1 (kinesin family member C1), also known as HSET (hematopoietic SH2 domain-containing protein), is a member of the kinesin-14 family, a group of microtubule-dependent motor proteins involved in intracellular transport and mitotic processes. Unlike most kinesins that move toward microtubule plus-ends, KIFC1 is a minus-end-directed motor, enabling unique roles in organizing microtubule networks and maintaining spindle integrity during cell division. It plays critical functions in mitosis, particularly in centrosome clustering, a mechanism vital for bipolar spindle formation in cells with supernumerary centrosomes, as seen in many cancers. Dysregulation of KIFC1 is linked to genomic instability, tumor progression, and chemoresistance, making it a potential therapeutic target.
Recombinant human KIFC1 protein is engineered using expression systems (e.g., *E. coli* or insect cells) to produce purified, functional protein for research. It typically retains ATPase and microtubule-binding activities, allowing in vitro studies on its mechanochemical properties, interactions with regulatory proteins, or drug screening. Structural analyses of recombinant KIFC1 have provided insights into its motor domain organization, cargo-binding regions, and mechanisms underlying its role in cancer cell survival. This protein serves as a key tool for exploring KIFC1’s contributions to cellular dynamics and developing inhibitors for anticancer therapies.
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