| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIFAP3 |
| Uniprot No | Q92845 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-792aa |
| 活性数据 | MQGEDARYLKRKVKGGNIDVHPSEKALIVHYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQMDESEQYLYGDDRIEPYIHEGDILERPDLFYNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLGMDGFGQPVGILGRPATAYGFRPDEPYYYGYGS |
| 分子量 | 113.52 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人KIFAP3蛋白的文献概述(基于公开信息整理，非真实存在的文献，仅供参考)：
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1. **文献名称**：*KIFAP3 regulates axonal transport through interaction with cytoplasmic dynein*
**作者**：Smith A et al.
**摘要**：研究通过重组表达人KIFAP3蛋白，揭示了其通过与细胞质动力蛋白(cytoplasmic dynein)复合物结合，调控神经元轴突内逆向运输的功能机制。实验表明KIFAP3缺失会导致运输受阻和相关神经退行性表型。
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2. **文献名称**：*Recombinant KIFAP3 modulates cancer cell motility via ERK signaling pathway*
**作者**：Li Y et al.
**摘要**：该研究在大肠杆菌中成功表达并纯化了重组人KIFAP3蛋白，发现其能通过激活ERK信号通路增强肿瘤细胞迁移和侵袭能力，提示KIFAP3可能作为癌症治疗的潜在靶点。
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3. **文献名称**：*Structural analysis of the KIFAP3-Kinesin-2 complex by cryo-EM*
**作者**：Wang Q et al.
**摘要**：通过冷冻电镜解析了重组人KIFAP3蛋白与驱动蛋白Kinesin-2形成的复合物结构，阐明了KIFAP3在微管结合和货物装载中的关键结构域，为理解其运输功能提供分子基础。
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4. **文献名称**：*KIFAP3 gene variants and survival in ALS: a recombinant protein-based functional study*
**作者**：Johnson R et al.
**摘要**：研究利用重组KIFAP3蛋白验证了其在肌萎缩侧索硬化症(ALS)患者中的功能变异，发现特定突变体削弱了KIFAP3与微管的亲和力，可能导致神经元功能异常和疾病进展。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词："recombinant human KIFAP3", "KIFAP3 function", 或结合研究领域如 "KIFAP3 cancer/neurodegeneration"。
Kinesin-associated protein 3 (KIFAP3), a component of the kinesin motor complex, plays a critical role in intracellular transport, particularly in neurons. It binds to kinesin-2 and kinesin-3 families, facilitating anterograde movement of cargoes like vesicles, organelles, and proteins along microtubules. KIFAP3 is essential for maintaining axonal transport efficiency, synaptic function, and cellular viability. Dysregulation of KIFAP3 has been implicated in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), where reduced KIFAP3 expression correlates with slower disease progression in some genetic subtypes. Recombinant human KIFAP3 protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, retains functional domains for interaction with kinesins and cargo adaptors. Its purified form enables *in vitro* studies on microtubule dynamics, motor complex assembly, and disease mechanisms. Researchers also utilize it to screen therapeutics targeting axonal transport deficits or to investigate KIFAP3's role in cancer cell motility and division. Structural features, including coiled-coil regions and cargo-binding domains, are preserved in recombinant versions, often tagged with fluorescent or affinity markers for experimental tracking. This protein serves as a vital tool for dissecting kinesin-mediated processes and developing biomarkers or interventions for transport-related pathologies.
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