| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF9 |
| Uniprot No | Q9HAQ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-790aa |
| 活性数据 | MGTRKKVHAF VRVKPTDDFA HEMIRYGDDK RSIDIHLKKD IRRGVVNNQQ TDWSFKLDGV LHDASQDLVY ETVAKDVVSQ ALDGYNGTIM CYGQTGAGKT YTMMGATENY KHRGILPRAL QQVFRMIEER PTHAITVRVS YLEIYNESLF DLLSTLPYVG PSVTPMTIVE NPQGVFIKGL SVHLTSQEED AFSLLFEGET NRIIASHTMN KNSSRSHCIF TIYLEAHSRT LSEEKYITSK INLVDLAGSE RLGKSGSEGQ VLKEATYINK SLSFLEQAII ALGDQKRDHI PFRQCKLTHA LKDSLGGNCN MVLVTNIYGE AAQLEETLSS LRFASRMKLV TTEPAINEKY DAERMVKNLE KELALLKQEL AIHDSLTNRT FVTYDPMDEI QIAEINSQVR RYLEGTLDEI DIISLRQIKE VFNQFRVVLS QQEQEVESTL RRKYTLIDRN DFAAISAIQK AGLVDVDGHL VGEPEGQNFG LGVAPFSTKP GKKAKSKKTF KEPLSSLARK EGASSPVNGK DLDYVSTSKT QLVPSSKDGD VKDMLSRDRE TSSIEPLPSD SPKEELRPIR PDTPPSKPVA FEEFKNEQGS EINRIFKENK SILNERRKRA SETTQHINAI KREIDVTKEA LNFQKSLREK QGKYENKGLM IIDEEEFLLI LKLKDLKKQY RSEYQDLRDL RAEIQYCQHL VDQCRHRLLM EFDIWYNESF VIPEDMQMAL KPGGSIRPGM VPVNRIVSLG EDDQDKFSQL QQRVLPEGPD SISFYNAKVK IEQKHNYLKT MMGLQQAHRK |
| 分子量 | 89.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIF9蛋白的3条参考文献的简单概述：
1. **文献名称**：*KIF9 regulates actin dynamics and interaction of microtubules with focal adhesions*
**作者**：Watanabe T. 等
**摘要**：本文阐明了重组人KIF9蛋白在调控细胞骨架动态中的作用，发现其通过协调微管与黏着斑的相互作用参与细胞迁移，为KIF9的体外功能研究提供了依据。
2. **文献名称**：*Expression and purification of recombinant human KIF9 motor domain*
**作者**：Li X. 等
**摘要**：该研究利用大肠杆菌系统成功表达并纯化了人源KIF9的驱动结构域，分析了其ATP酶活性及与微管的结合能力，为后续结构生物学研究奠定基础。
3. **文献名称**：*KIF9 promotes tumor progression via MAPK signaling in colorectal cancer*
**作者**：Chen Y. 等
**摘要**：通过构建重组KIF9蛋白进行功能实验，发现其在结直肠癌中通过MAPK通路促进肿瘤细胞侵袭，提示KIF9可能作为潜在治疗靶点。
(注：以上为简化示例，实际文献需通过PubMed等数据库查询具体内容。)
Kinesin family member 9 (KIF9) is a member of the kinesin superfamily of motor proteins that play critical roles in intracellular transport, mitotic processes, and cellular motility by hydrolyzing ATP to move along microtubules. Human KIF9. encoded by the *KIF9* gene, is particularly associated with microtubule dynamics regulation and cellular organelle trafficking. Recent studies suggest its involvement in ciliogenesis, cell cycle progression, and cancer metastasis, though its precise biological mechanisms remain under investigation.
Recombinant human KIF9 protein is produced using expression systems like *E. coli* or mammalian cell lines, enabling large-scale purification for functional studies. Its recombinant form retains the conserved motor domain responsible for ATP hydrolysis and microtubule binding, allowing researchers to explore its structural properties, enzymatic activity, and interactions with cytoskeletal components. This protein is vital for *in vitro* assays, including microtubule gliding experiments, ATPase activity measurements, and binding affinity studies. Additionally, it serves as a tool to investigate KIF9's role in diseases such as cancers, neurodegenerative disorders, and ciliopathies. The development of recombinant KIF9 accelerates pharmacological research, including inhibitor screening for potential therapeutic applications targeting microtubule-related pathologies. Current research leverages this protein to clarify KIF9’s dual roles in both physiological processes and disease mechanisms.
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