| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF3B |
| Uniprot No | O15066 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-747aa |
| 活性数据 | MSKLKSSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKNPKGTAHEMPKTFTFDAVYDWNAKQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTYTMEGIRGDPEKRGVIPNSFDHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQTKRLELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSEVGLDGENHIRVGKLNLVDLAGSERQAKTGAQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKAQLEKRSIGRRKRREKRREGGGSGGGGEEEEEEGEEGEEEGDDKDDYWREQQEKLEIEKRAIVEDHSLVAEEKMRLLKEKEKKMEDLRREKDAAEMLGAKIKAMESKLLVGGKNIVDHTNEQQKILEQKRQEIAEQKRREREIQQQMESRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQAVKAEIHDLQEEHIKERQELEQTQNELTRELKLKHLIIENFIPLEEKSKIMNRAFFDEEEDHWKLHPITRLENQQMMKRPVSAVGYKRPLSQHARMSMMIRPEARYRAENIVLLELDMPSRTTRDYEGPAIAPKVQAALDAALQDEDEIQVDASSFESTANKKSKARPKSGRKSGSSSSSSGTPASQLYPQSRGLVPK |
| 分子量 | 111.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性示例(非真实文献，仅供格式参考)：
1. **文献名称**：*KIF3B重组蛋白在纤毛形成中的分子机制研究*
**作者**：Li et al.
**摘要**：本研究成功表达并纯化重组人KIF3B蛋白，通过体外实验验证其与KAP3蛋白复合物的相互作用，揭示其在纤毛组装中的关键作用。
2. **文献名称**：*重组KIF3B驱动蛋白的ATP酶活性分析*
**作者**：Yamamoto et al.
**摘要**：通过重组KIF3B蛋白与微管结合实验，发现其ATP水解活性对胞内物质运输的调控机制，为神经细胞轴突运输障碍提供新见解。
3. **文献名称**：*KIF3B重组蛋白在肿瘤迁移中的功能探索*
**作者**：Chen et al.
**摘要**：利用重组KIF3B蛋白进行细胞侵袭实验，证明其通过Wnt/β-catenin通路抑制肺癌细胞转移，提示其潜在抑癌作用。
4. **文献名称**：*重组KIF3B与Bardet-Biedl综合征相关蛋白的互作网络*
**作者**：Smith et al.
**摘要**：通过重组蛋白共沉淀技术，揭示KIF3B与BBS蛋白复合物的协同作用，阐明了纤毛病致病机制中KIF3B的分子缺失效应。
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如需真实文献，建议通过PubMed或Web of Science以“KIF3B recombinant”或“KIF3B function”为关键词检索。
Kinesin family member 3B (KIF3B) is a motor protein belonging to the kinesin-2 superfamily, critical for intracellular transport along microtubules. In humans, KIF3B forms a heterodimeric complex with KIF3A or interacts with kinesin-associated protein 3 (KAP3) to facilitate cargo movement, including membranous organelles, vesicles, and mRNA. It plays pivotal roles in ciliogenesis, cell polarity, and signal transduction by ensuring proper trafficking of molecular components. Dysregulation of KIF3B is linked to ciliopathies, neurodegenerative disorders, and cancer progression.
Recombinant human KIF3B protein is engineered through bacterial or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. Its production enables studies on microtubule-dependent transport mechanisms, interactions with cargo adaptors, and structural insights into kinesin motor domains. Researchers utilize this protein to investigate cellular processes like mitotic spindle formation, neuronal axon transport, and ciliary assembly in disease models. Additionally, it serves as a tool for drug screening targeting transport-related pathologies or cilia-associated disorders. The availability of recombinant KIF3B accelerates functional genomics and therapeutic development in intracellular trafficking research.
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