| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF3A |
| Uniprot No | Q9Y496 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-702aa |
| 活性数据 | MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYKQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPESKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGIIPNSFAHIFGHIAKAEGDTRFLVRVSYLGIYNEEVRDLLGKDQTQRLEVIERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGIDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDPKDALLRQFQKEIEELKKKLEEGEEISGSDISGSEEDDDEEGEVGEDGEKRKKRRDQTGKKKVSPDKMIEMQAKIDEERKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKTGRRKRSAKPETVIDSLLQ |
| 分子量 | 106.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIF3A蛋白的3篇文献概览：
1. **文献名称**: **"KIF3A is a new microtubule-based anterograde motor in the nerve axon"**
**作者**: Kikkawa M, et al.
**摘要**: 该研究阐明了KIF3A作为驱动蛋白家族成员的功能，利用重组人KIF3A蛋白验证其在神经轴突中的微管依赖的顺向运输作用，揭示了其与KIF3B形成异源二聚体参与囊泡运输的分子机制。
2. **文献名称**: **"Reconstitution of recombinant human KIF3A-KAP3 complexes and their role in ciliogenesis"**
**作者**: Yamazaki H, et al.
**摘要**: 通过重组技术表达了人KIF3A及适配蛋白KAP3.体外实验证明两者复合体是纤毛形成的关键驱动因子，为纤毛相关疾病(如多囊肾病)的机制研究提供了分子基础。
3. **文献名称**: **"Functional analysis of KIF3A mutations in human developmental disorders"**
**作者**: Brown NJ, et al.
**摘要**: 利用重组KIF3A蛋白结合CRISPR技术，揭示了该蛋白突变导致胚胎发育异常及神经管缺陷的病理机制，强调了其在内质网至高尔基体膜泡运输中的关键作用。
注：以上文献信息为示例性内容，实际引用需根据真实文献调整。建议通过PubMed或Google Scholar以关键词“recombinant human KIF3A”检索最新研究。
KIF3A, a member of the kinesin-2 family, is a motor protein involved in intracellular transport along microtubules. It functions as part of a heterodimeric complex with KIF3B, partnering with kinesin-associated protein 3 (KAP3) to form the KIF3A/KIF3B/KAP3 complex. This complex facilitates anterograde transport of cargo, such as membrane organelles, signaling molecules, and protein complexes, contributing to critical cellular processes like cilia formation, cell division, and signal transduction. KIF3A is essential for the assembly and maintenance of primary cilia, specialized organelles regulating Hedgehog and Wnt signaling pathways. Dysregulation of KIF3A is linked to ciliopathies, cancers, and neurological disorders.
Recombinant human KIF3A protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, retains structural and functional integrity for *in vitro* studies. It enables mechanistic exploration of KIF3A’s roles in cargo binding, motor activity, and interactions with regulatory proteins. Applications include dissecting transport mechanisms, screening drug candidates targeting cilia-related diseases, and modeling pathological processes. Often purified with affinity tags (e.g., His-tag), recombinant KIF3A supports structural studies, antibody development, and functional assays, aiding research into cellular transport biology and therapeutic strategies for KIF3A-associated disorders. Its study advances understanding of microtubule-based motility and ciliary dysfunction mechanisms.
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