关键信息
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基因名
POLM
- 应用
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别名
POLM；polmu；DNA-directed DNA/RNA polymerase mu
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NP87
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表达区间
1-494aa
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氨基酸序列
MLPKRRRARVGSPSGDAASSTPPSTRFPGVAIYLVEPRMGRSRRAFLTGLARSKGFRVLDACSSEATHVVMEETSAEEAVSWQERRMAAAPPGCTPPALLDISWLTESLGAGQPVPVECRHRLEVAGPRKGPLSPAWMPAYACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLPHFGEHSSRVVQELLEHGVCEEVERVRRSERYQTMKLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAGLQHHQDLSTPVLRSDVDALQQVVEEAVGQALPGATVTLTGGFRRGKLQGHDVDFLITHPKEGQEAGLLPRVMCRLQDQGLILYHQHQHSCCESPTRLAQQSHMDAFERSFCIFRLPQPPGAAVGGSTRPCPSWKAVRVDLVVAPVSQFPFALLGWTGSKLFQRELRRFSRKEKGLWLNSHGLFDPEQKTFFQAASEEDIFRHLGLEYLPPEQRNA
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分子量
58.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
POLM, or DNA Polymerase Mu, is a member of the DNA polymerase family, which plays a vital role in DNA repair and synthesis processes within cells. Discovered in the late 1990s, POLM is particularly notable for its ability to perform non-homologous end joining (NHEJ), a critical pathway for repairing double-strand breaks in DNA, which can arise from various sources such as ionizing radiation, chemotherapy, or oxidative stress. Unlike other polymerases, POLM possesses unique features, including a terminal deoxynucleotidyl transferase activity, which allows it to add nucleotides to the ends of broken DNA, facilitating the repair process. This function is crucial for maintaining genomic stability and preventing mutations that could lead to cancer. Research has shown that POLM is involved in the repair of immunoglobulin gene segments during lymphocyte development and plays a role in V(D)J recombination, a process essential for generating diverse antibody repertoires. Furthermore, studies have indicated that dysregulation of POLM activity may contribute to tumorigenesis, making it a potential target for therapeutic intervention. Understanding the structural and functional properties of POLM, along with its involvement in DNA repair mechanisms, is critical for developing strategies to enhance genomic integrity and treat diseases related to DNA damage, including cancer. Given its importance in these cellular processes, POLM continues to be a subject of intensive research aimed at elucidating its molecular mechanisms and potential applications in clinical settings.
