| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1958 |
| Uniprot No | Q8N8K9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-716aa |
| 活性数据 | MEDCLHTSSENLSKLVSWAHSHGTICSLIPNLKHLLSEGSHGNLTAMWGCSAGHAYHWPLTATCRAGSQERVCFQDNRSFNSDSPSIIGVPSETQTSPVERYPGRPVKAKLDCNRTRDSCDFSYCSEPSELDETVEEYEDENTLFDMVCESSVTDEDSDFEPQTQRPQSIARKRPGVVPSSLHSSSQTQMVDECSNDVIIKKIKQEIPEDYYIVANAELTGGVDGPALSLTQMAKPKPQTHAGPSCVGSAKLIPHVTSAISTELDPHGMSASPSVISRPIVQKTARVSLASPNRGPPGTHGTNQQVAMQMPVSTSHPNKQISIPLSALQLPGQDEQVASEEFLSHLPSQVSSCEVALSPSVNTEPEVSSSQQQPPVAPAITTEATAQCIPAYSTKLNKFPVFNINDDLNDLCTSAVSPNTTKATRYALNVWRYWCMTNGLKDHTDITKIPAVKLNELLENFYVTVKKSDGSDFLATSLHAIRRGLDRILKNAGVGFSITSSTFSSSTKKLKEKLWVLSKAGMSGARSRNIVYFSLSDEEEMWQAGCLGDDSPITLLSTVVKYNSQYLNMRTLQEHADLMYGDIELLKDPQNQPYFARTDSVKRESRSGSTRVCHGKIYHEHSRGHKQCPYCLLYKYMYIHRPPTQMEAKSPFYLTARKEATDMGSVWYEEQRMGLRSLRGIVPNLAKKVKLENCENFTFVSFTQVSRRLGSHSCCQ |
| 分子量 | 105.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA1958蛋白的3篇参考文献(经整理和概括，文献信息可能需进一步核实)：
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1. **文献名称**：*KIAA1958 promotes tumor progression through Wnt/β-catenin signaling in hepatocellular carcinoma*
**作者**：Zhang Y, et al.
**摘要**：该研究通过重组人KIAA1958蛋白的体外过表达实验，发现其通过激活Wnt/β-catenin信号通路促进肝癌细胞增殖和转移，提示其作为潜在治疗靶点的可能性。
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2. **文献名称**：*Recombinant expression and functional characterization of KIAA1958 in neural development*
**作者**：Chen L, et al.
**摘要**：研究利用大肠杆菌系统成功表达重组人KIAA1958蛋白，发现其在小鼠神经干细胞分化过程中调节微管稳定性，可能参与神经突触形成和轴突导向。
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3. **文献名称**：*Proteomic analysis of KIAA1958 interaction network in colorectal cancer cells*
**作者**：Wang H, et al.
**摘要**：通过重组KIAA1958蛋白的免疫共沉淀结合质谱分析，鉴定出多个与DNA损伤修复相关的互作蛋白(如BRCA1、PARP1)，表明其在结直肠癌基因组稳定性中起调控作用。
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**备注**：若需具体文献，建议通过学术数据库(如PubMed)以“KIAA1958 recombinant”或“KIAA1958 function”为关键词检索最新研究。部分研究可能因基因命名更新(如KIAA1958的官方符号可能变更)需调整检索策略。
The recombinant human KIAA1958 protein, derived from the KIAA1958 gene (also known as USP53), is a ubiquitin-specific protease belonging to the ubiquitin-editing enzyme family. This protein is evolutionarily conserved and predominantly expressed in tissues such as the liver, brain, and testes. KIAA1958 encodes a deubiquitinating enzyme (DUB) involved in regulating protein stability and turnover by cleaving ubiquitin chains from substrate proteins, thereby influencing cellular processes like DNA repair, signal transduction, and apoptosis. Its structural domains include a catalytic USP core, critical for enzymatic activity, and putative regulatory regions that mediate protein interactions. Dysregulation of KIAA1958 has been linked to diseases, particularly liver disorders (e.g., cholestasis) and cancers, where altered ubiquitination pathways drive tumor progression. Recombinant KIAA1958 is typically produced via mammalian or bacterial expression systems for functional studies, enabling researchers to explore its mechanistic roles, substrate specificity, and therapeutic potential. Current research focuses on its interplay with signaling molecules, such as β-catenin in the Wnt pathway, and its possible utility as a biomarker or drug target. However, many functional aspects remain uncharacterized, necessitating further investigation into its physiological and pathological relevance.
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