| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1715 |
| Uniprot No | Q9C0E8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-428aa |
| 活性数据 | MGGLFSRWRTKPSTVEVLESIDKEIQALEEFREKNQRLQKLWVGRLILYSSVLYLFTCLIVYLWYLPDEFTARLAMTLPFFAFPLIIWSIRTVIIFFFSKRTERNNEALDDLKSQRKKILEEVMEKETYKTAKLILERFDPDSKKAKECEPPSAGAAVTARPGQEIRQRTAAQRNLSPTPASPNQGPPPQVPVSPGPPKDSSAPGGPPERTVTPALSSNVLPRHLGSPATSVPGMGLHPPGPPLARPILPRERGALDRIVEYLVGDGPQNRYALICQQCFSHNGMALKEEFEYIAFRCAYCFFLNPARKTRPQAPRLPEFSFEKRQVVEGSSSVGPLPSGSVLSSDNQFNEESLEHDVLDDNTEQTDDKIPATEQTNQVIEKASDSEEPEEKQETENEEASVIETNSTVPGADSIPDPELSGESLTAE |
| 分子量 | 74.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为根据要求生成的3条关于重组人KIAA1715蛋白的虚构参考文献示例(请注意这些文献为模拟创作，实际研究中请以真实论文为准)：
1. **"Expression and purification of recombinant human KIAA1715 protein in E. coli system"**
- **作者**: Kim, J. et al. (2020)
- **摘要**: 本研究成功利用大肠杆菌表达系统实现了人源KIAA1715蛋白的重组表达，通过His标签亲和层析纯化获得高纯度蛋白。Western blot验证其分子量约为180 kDa，圆二色谱分析显示其具有α-螺旋和β-折叠复合结构，为后续功能研究奠定基础。
2. **"KIAA1715 regulates histone H3K4 demethylation during neural stem cell differentiation"**
- **作者**: Zhang, L. et al. (2018)
- **摘要**: 文章揭示了重组KIAA1715蛋白在神经干细胞分化中的表观遗传调控作用。通过体外甲基化实验证明，该蛋白与LSD1协同作用，特异性催化组蛋白H3K4me2/me1的去甲基化过程，激活下游神经发育相关基因(如SOX2)的表达。
3. **"Proteomic identification of KIAA1715-interacting proteins in hepatocellular carcinoma"**
- **作者**: Smith, R. et al. (2021)
- **摘要**: 运用质谱分析技术，筛选出重组KIAA1715蛋白在肝癌细胞中与HDAC1、DNMT3A等表观调控因子存在物理相互作用。功能实验显示其过表达显著抑制HepG2细胞增殖(P<0.01)，提示其可能通过表观遗传通路发挥肿瘤抑制作用。
注：以上文献信息均为示例性虚构内容，实际文献需通过PubMed、Web of Science等学术数据库查询真实研究论文。
Recombinant human KIAA1715 protein, encoded by the *KIAA1715* gene (also known as *HDHD5*), is a less-studied protein with emerging roles in cellular processes. Located on chromosome 1p36.13. the gene produces a protein containing conserved domains, including DUF1983 and HEAT repeats, which are implicated in protein-protein interactions and structural scaffolding. KIAA1715 is ubiquitously expressed, with higher levels in tissues like the brain, suggesting potential neurological functions.
Though its precise mechanism remains unclear, KIAA1715 is proposed to regulate transcription, chromatin remodeling, or metabolic pathways. Studies link it to hydrolase activity, possibly influencing glycerophospholipid metabolism, and it may interact with Wnt signaling components, affecting development and disease. Altered KIAA1715 expression has been observed in neurodevelopmental disorders, cancers, and psychiatric conditions, though causal relationships require validation.
Recombinant KIAA1715 is typically produced in *E. coli* or mammalian systems, fused with tags (e.g., His-tag) for purification and functional studies. Its recombinant form enables biochemical assays, antibody development, and exploration of molecular interactions. Despite progress, KIAA1715's full functional landscape and therapeutic potential remain underexplored, necessitating further mechanistic studies to clarify its physiological and pathological roles.
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