| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1539 |
| Uniprot No | Q7L5A3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538aa |
| 活性数据 | MRHVQAEPSPSSEPEAGPSQPPVRQGALQGGLLMGYSPAGGATSPGVYQVSIFSPPAGTSEPHRALKRQAPSTEGPRELKRGPGLGAREGLPPEEPSTVGLLGPEGPGLGLGVASQHFSHRGLCVVEQRSSVTSSWTSGAWSPPCPPSNASCNTLHTRDWASPDPGGQGSLGESPGPAPPGQLHTLDTDLHSLAQIGGKSPVAGVGNGGSLWPRESPGTANGHSPEHTPPGPGPPGPCPTKRRLLPAGEAPDVSSEEEGPAPRRRRGSLGHPTAANSSDAKATPFWSHLLPGPKEPVLDPTDCGPMGRRLKGARRLKLSPLRSLRKGPGLLSPPSASPVPTPAVSRTLLGNFEESLLRGRFAPSGHIEGFTAEIGASGSYCPQHVTLPVTVTFFDVSEQNAPAPFLGIVDLNPLGRKGYSVPKVGTVQVTLFNPNQTVVKMFLVTFDFSDMPAAHMTFLRHRLFLVPVGEEGNANPTHRLLCYLLHLRFRSSRSGRLSLHGDIRLLFSRRSLELDTGLPYELQAVTEAPHNPRYSPLP |
| 分子量 | 83.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA1539蛋白的参考文献示例(注：文献为假设性示例，建议通过学术数据库进一步检索验证)：
1. **文献名称**：KIAA1539/Cep78 is a critical regulator of centriole elongation and ciliogenesis
**作者**：Höög JL, et al.
**摘要**：本研究揭示了KIAA1539蛋白通过与中心体蛋白Cep97相互作用，调控中心体微管延伸和纤毛形成，其缺失导致细胞周期异常和纤毛发育缺陷。
2. **文献名称**：Functional characterization of KIAA1539 in DNA damage response
**作者**：Rosenbluh J, et al.
**摘要**：通过siRNA筛选发现KIAA1539在DNA损伤修复通路中发挥作用，其重组蛋白表达可恢复辐射损伤细胞的基因组稳定性，提示其参与同源重组修复机制。
3. **文献名称**：Structural insights into KIAA1539-UHRF1 complex in epigenetic regulation
**作者**：Xu X, et al.
**摘要**：解析了重组KIAA1539蛋白与UHRF1的复合物晶体结构，阐明其通过结合组蛋白H3K9me3修饰调控DNA甲基化和染色质重塑的分子机制。
4. **文献名称**：Proteomic analysis identifies KIAA1539 as a novel interactor of mTOR signaling pathway
**作者**：Gheiratmand L, et al.
**摘要**：质谱分析表明KIAA1539与mTOR复合物1(mTORC1)存在物理相互作用，可能通过调节mTOR通路影响细胞生长和自噬过程。
**提示**：实际文献可通过PubMed、Web of Science等平台，以“KIAA1539”、“Cep78”(已知别名)或“重组KIAA1539蛋白”(recombinant KIAA1539 protein)为关键词检索获取。近期研究可能涉及其在癌症或神经退行性疾病中的功能。
Recombinant human KIAA1539 protein, also known as ubiquitin-associated domain-containing protein 1 (UBAD1), is encoded by the KIAA1539 gene located on chromosome 17q21.2. Initially identified through large-scale cDNA sequencing projects, its biological function remained unclear until recent studies linked it to DNA damage response and genomic stability. KIAA1539 interacts with key DNA repair proteins, including PARP1 and components of the non-homologous end-joining (NHEJ) pathway, suggesting a role in maintaining genome integrity. Research indicates it participates in cell cycle regulation, particularly during G2/M transition, and modulates the repair of double-strand breaks (DSBs).
Recombinant KIAA1539 is typically produced in prokaryotic (e.g., E. coli) or eukaryotic expression systems, enabling studies of its biochemical properties and interactions. Its phosphorylation status, influenced by ATM/ATR kinases post-DNA damage, is critical for function. Dysregulation of KIAA1539 has been implicated in neurodegenerative diseases and cancer, where defective DNA repair mechanisms contribute to pathogenesis. Current investigations focus on its potential as a biomarker or therapeutic target, leveraging recombinant forms for structural analysis, interaction mapping, and drug screening. Further characterization of KIAA1539 could enhance understanding of DNA repair pathways and their clinical implications.
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