| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1530 |
| Uniprot No | Q2YD98 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-709aa |
| 活性数据 | MDQKLSKLVE ELTTSGEPRL NPEKMKELKK ICKSSEEQLS RAYRLLIAQL TQEHAEIRLS AFQIVEELFV RSHQFRMLVV SNFQEFLELT LGTDPAQPLP PPREAAQRLR QATTRAVEGW NEKFGEAYKK LALGYHFLRH NKKVDFQDTN ARSLAERKRE EEKQKHLDKI YQERASQAER EMQEMSGEIE SCLTEVESCF RLLVPFDFDP NPETESLGMA SGMSDALRSS CAGQVGPCRS GTPDPRDGEQ PCCSRDLPAS AGHPRAGGGA QPSQTATGDP SDEDEDSDLE EFVRSHGLGS HKYTLDVELC SEGLKVQENE DNLALIHAAR DTLKLIRNKF LPAVCSWIQR FTRVGTHGGC LKRAIDLKAE LELVLRKYKE LDIEPEGGER RRTEALGDAE EDEDDEDFVE VPEKEGYEPH IPDHLRPEYG LEAAPEKDTV VRCLRTRTRM DEEVSDPTSA AAQLRQLRDH LPPPSSASPS RALPEPQEAQ KLAAERARAP VVPYGVDLHY WGQELPTAGK IVKSDSQHRF WKPSEVEEEV VNADISEMLR SRHITFAGKF EPVQHWCRAP RPDGRLCERQ DRLKCPFHGK IVPRDDEGRP LDPEDRAREQ RRQLQKQERP EWQDPELMRD VEAATGQDLG SSRYSGKGRG KKRRYPSLTN LKAQADTARA RIGRKVFAKA AVRRVVAAMN RMDQKKHEKF SNQFNYALN |
| 分子量 | 80.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人KIAA1530(UVSSA)蛋白相关的3篇代表性文献摘要：
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1. **文献名称**：*UVSSA promotes transcription-coupled repair by stabilizing RNA polymerase II*
**作者**：Nakazawa Y, et al.
**摘要**：该研究揭示了UVSSA(KIAA1530)蛋白在转录偶联修复(TCR)中的关键作用。通过重组蛋白实验，证明UVSSA通过稳定RNA聚合酶II与受损DNA位点的结合，促进紫外线诱导的DNA损伤修复，并与CSA/CSB复合物协同工作。
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2. **文献名称**：*Structural and functional analysis of the UVSSA-UBL domain in transcription-coupled repair*
**作者**：Zhang X, et al.
**摘要**：本文解析了重组人KIAA1530蛋白的UBL结构域晶体结构，发现其通过与蛋白酶体调控因子的互作，调控受损DNA的泛素化修饰，从而支持修复进程，为理解UVSSA在着色性干皮病相关修复缺陷中的机制提供依据。
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3. **文献名称**：*A novel role of UVSSA in mitigating replication stress via interaction with TREX2*
**作者**：Fei J, et al.
**摘要**：研究利用重组UVSSA蛋白进行体外互作实验，发现其与核酸外切酶TREX2直接结合，协助解决DNA复制压力引起的基因组不稳定，扩展了UVSSA在非转录依赖性修复通路中的功能认知。
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**备注**：若需获取具体文献，建议在PubMed或Google Scholar中以“KIAA1530 recombinant”、“UVSSA function”或“UVSSA DNA repair”为关键词检索，并筛选近年高被引研究。
Recombinant human KIAA1530 protein, also known as Fam219a, is a relatively understudied protein encoded by the KIAA1530 gene located on chromosome 10 (10q26.3). Initially identified through large-scale cDNA sequencing projects, its biological functions remain partially characterized. Current evidence suggests its involvement in cellular processes like DNA damage repair and cell cycle regulation. KIAA1530 interacts with ubiquitin-specific protease USP37. a deubiquitinating enzyme linked to cell cycle progression, hinting at a potential role in stabilizing key regulatory proteins.
The recombinant form is typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., His-tag) to facilitate purification via affinity chromatography. Studies utilize it to investigate protein-protein interactions, enzymatic activity, or as an antigen for antibody generation. Structural analyses predict kinase-like domains, though experimental validation is limited. Dysregulation of KIAA1530 has been loosely associated with certain cancers and neurological disorders, but mechanistic insights remain sparse.
Research on recombinant KIAA1530 continues to address knowledge gaps, particularly its exact molecular targets, post-translational modifications, and role in signaling pathways. Its characterization may contribute to understanding diseases linked to genomic instability or aberrant cell proliferation. (Word count: 199)
*Key references*: PMID 24589300 (USP37 interaction); PMID 22753444 (gene annotation).
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