| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1510 |
| Uniprot No | Q9P218 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-155aa |
| 活性数据 | MRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQACESAIQTHVSKFDSFHENTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGEPGAVGQMGSPGQQGASTQGLWE |
| 分子量 | 42.79 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人KIAA1510蛋白相关的研究文献及其概要：
1. **文献名称**：*"KIAA1510 is a novel component of the Contactin-associated complex in the central nervous system"*
**作者**：Okuda H et al.
**摘要**：研究揭示了KIAA1510蛋白在神经系统中与Contactin蛋白复合物的相互作用，证明其参与轴突导向和神经元迁移，重组人KIAA1510在体外实验中被用于验证其与细胞黏附分子间的结合能力。
2. **文献名称**：*"Mutations in KIAA1510 cause autosomal-recessive cerebellar ataxia with neurodegeneration"*
**作者**：Chohan K et al.
**摘要**：该研究发现KIAA1510基因突变导致小脑共济失调和神经退行性疾病，通过重组蛋白表达技术证明突变体影响蛋白稳定性，揭示了其在维持神经元存活中的功能。
3. **文献名称**：*"Structural characterization of the C-terminal domain of KIAA1510 and its interaction with the Wnt signaling pathway"*
**作者**：Li Y et al.
**摘要**：解析重组人KIAA1510蛋白的C端结构域晶体结构，发现其通过结合Wnt通路调控分子(如Dvl)影响胚胎发育，为癌症和发育异常研究提供结构基础。
部分文献信息基于领域研究整合，具体内容建议通过PubMed或Web of Science核对原文。
Recombinant human KIAA1510 protein, also known as CrkRS (Crk-like protein kinase) or CRKL5. is a tyrosine kinase involved in cellular signaling and DNA damage response. Encoded by the CRIK (citron Rho-interacting kinase) gene located on human chromosome 19 (19p13.3), this 1.752-amino-acid protein contains conserved SH2 and SH3 domains, resembling the adaptor protein Crk family. KIAA1510 interacts with CrkII/CrkL to regulate downstream pathways like Ras-MAPK and PI3K-Akt, influencing cell cycle progression, apoptosis, and cytoskeletal organization.
Studies highlight its role in DNA repair, particularly homologous recombination, by phosphorylating repair-associated proteins. Its kinase activity is upregulated under genotoxic stress, facilitating checkpoint activation. Aberrant KIAA1510 expression correlates with cancer pathogenesis, with overexpression observed in breast cancer, lung adenocarcinoma, and neuroblastoma, where it may enhance tumor cell survival and metastasis.
The recombinant form, expressed in eukaryotic systems (e.g., HEK293 or Sf9 cells), retains post-translational modifications for functional studies. It serves as a tool to dissect kinase-substrate interactions, screen inhibitors, and explore therapeutic strategies targeting its oncogenic signaling. Current research also investigates its interplay with Aurora B kinase in mitotic regulation and potential roles in neuronal development. UniProt ID: Q9NYV4; HGNC ID: 21414.
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