| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA1394 |
| Uniprot No | A5YM72 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385aa |
| 活性数据 | MTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAALRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK |
| 分子量 | 68.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人KIAA1394蛋白相关的虚构参考文献示例(仅供参考，实际文献请通过学术数据库验证)：
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1. **文献名称**: "KIAA1394 promotes tumor invasion via regulating MMP-2/9 expression in hepatocellular carcinoma"
**作者**: Li X., Wang Y., Chen Z.
**摘要**: 本研究通过重组表达人KIAA1394蛋白，发现其能够通过激活ERK信号通路显著上调肝癌细胞中基质金属蛋白酶(MMP-2/9)的表达，从而增强癌细胞迁移和侵袭能力。蛋白纯化采用大肠杆菌表达系统和镍柱亲和层析法。
2. **文献名称**: "Structural characterization of recombinant human KIAA1394 and its role in neuronal apoptosis"
**作者**: Zhang H., Tanaka K., Liu R.
**摘要**: 利用杆状病毒-昆虫细胞系统重组表达KIAA1394蛋白，并通过X射线晶体学解析其N端结构域的三维结构。实验表明该蛋白在缺氧条件下通过结合Bax蛋白调控神经元凋亡，为阿尔茨海默病机制研究提供新线索。
3. **文献名称**: "KIAA1394 interacts with HSP90 to modulate protein stability in colorectal cancer"
**作者**: Gupta S., Kim M., Park J.
**摘要**: 本研究通过哺乳动物HEK293细胞表达重组人KIAA1394蛋白，证实其与分子伴侣HSP90直接相互作用，并稳定多种致癌蛋白。敲低KIAA1394导致HSP90底物蛋白降解，抑制结直肠肿瘤生长。
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**注意**：以上文献为根据领域研究热点生成的示例，实际研究中请以真实发表的文献为准。建议通过PubMed、Web of Science等平台检索关键词“KIAA1394”、“recombinant protein”或“HIAT1”(该基因别名)获取准确信息。
Recombinant human KIAA1394 protein is a product of the KIAA1394 gene, initially identified through large-scale sequencing projects aimed at characterizing unannotated transcripts in the human genome. This gene, located on chromosome X (Xp11.3), encodes a protein of approximately 1.500 amino acids with a predicted molecular weight of ~170 kDa. Although its exact biological function remains incompletely characterized, structural analyses suggest KIAA1394 contains several coiled-coil domains and a conserved DUF4268 region, hinting at potential roles in protein-protein interactions or intracellular scaffolding.
Recombinant production of KIAA1394 typically employs expression systems like mammalian cells or bacteria, enabling biochemical and functional studies. Limited evidence links KIAA1394 to cellular stress responses, metabolism, or cancer progression, though these associations require validation. Its overexpression in certain tumors and interaction partners (e.g., kinases, ubiquitin-related proteins) implies involvement in signaling pathways or post-translational regulation. Current research focuses on clarifying its physiological substrates, disease relevance, and utility as a biomarker or therapeutic target. The availability of recombinant KIAA1394 protein facilitates antibody development, structural studies, and high-throughput screening to unravel its mechanistic contributions to cellular processes. Further functional proteomics and knockout models are needed to define its role in health and disease.
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