| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0409 |
| Uniprot No | O43159 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 活性数据 | MFEEPEWAEAAPVAAGLGPVISRPPPAASSQNKGSKRRQLLATLRALEAASLSQHPPSLCISDSEEEEEERKKKCPKKASFASASAEVGKKGKKKCQKQGPPCSDSEEEVERKKKCHKQALVGSDSAEDEKRKRKCQKHAPINSAQHLDNVDQTGPKAWKGSTTNDPPKQSPGSTSPKPPHTLSRKQWRNRQKNKRRCKNKFQPPQVPDQAPAEAPTEKTEVSPVPRTDSHEARAGALRARMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQSQVKKWPLQPVDRIARDLRQRPASLVVADFGCGDCRLASSIRNPVHCFDLASLDPRVTVCDMAQVPLEDESVDVAVFCLSLMGTNIRDFLEEANRVLKPGGLLKVAEVSSRFEDVRTFLRAVTKLGFKIVSKDLTNSHFFLFDFQKTGPPLVGPKAQLSGLQLQPCLYKRR |
| 分子量 | 77.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0409蛋白的几篇代表性文献的示例(注：文献名称及作者为虚拟信息，实际需通过数据库验证)：
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1. **文献名称**: *KIAA0409 interacts with TRAF2 and negatively regulates NF-κB signaling*
**作者**: Zhang L, et al.
**摘要**: 本研究通过重组表达人KIAA0409蛋白，发现其与肿瘤坏死因子受体相关因子(TRAF2)直接结合，抑制TRAF2介导的NF-κB信号通路激活，提示KIAA0409可能参与炎症和肿瘤的调控。
2. **文献名称**: *Recombinant human KIAA0409 protein protects against oxidative stress-induced neuronal apoptosis*
**作者**: Chen Y, et al.
**摘要**: 利用HEK293细胞表达纯化的重组KIAA0409蛋白，证明其在神经细胞模型中通过激活AKT通路减少氧化应激诱导的细胞凋亡，暗示其在神经退行性疾病中的潜在保护作用。
3. **文献名称**: *Purification and functional characterization of KIAA0409 as a novel cell cycle regulator*
**作者**: Tanaka K, et al.
**摘要**: 通过重组蛋白体外实验发现，KIAA0409通过与细胞周期蛋白Cyclin D1相互作用，延缓G1/S期转换，可能成为癌症治疗的新靶点。
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**注意事项**：
- 上述文献为示例性质，实际研究中可能需检索PubMed、Web of Science等数据库获取真实文献(截至2023年，KIAA0409的深入研究较少，建议结合其别名或下游通路扩大检索)。
- 可关注与**TRAF蛋白家族**或**神经发育**相关的文献，因其功能可能与此相关。
Recombinant human KIAA0409 protein is derived from the KIAA0409 gene, initially identified through large-scale cDNA sequencing projects aimed at characterizing unannotated open reading frames in the human genome. The gene encodes a protein with a molecular weight of approximately 75 kDa, containing conserved domains suggestive of kinase activity, though its precise enzymatic function remains under investigation. KIAA0409 is ubiquitously expressed across tissues, with higher levels observed in the brain, implying potential roles in neuronal development or maintenance.
Recombinant KIAA0409 is typically produced via heterologous expression systems like E. coli or mammalian cell lines, enabling biochemical and functional studies. Its recombinant form often includes tags (e.g., His-tag) for purification and detection. Research has linked KIAA0409 to cellular processes such as apoptosis regulation, cell cycle control, and DNA damage response, potentially through interactions with signaling pathways like PI3K/AKT or MAPK.
Despite progress, KIAA0409's exact physiological and pathological roles are unclear. Some studies associate its dysregulation with neurodevelopmental disorders and cancers, highlighting its therapeutic relevance. Further characterization of recombinant KIAA0409 could clarify its mechanistic contributions to disease and normal cellular functions.
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