| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0368 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519aa |
| 活性数据 | MDSARLSAAKSSPMMETINMCLQYLDVSVLGELVPRLCELIRSGVGLGTKGGCASVIVSLTTQCPQDLTPYSGKLMSALLSGLTDRNSVIQKSCAFAMGHLVRTSRDSSTEKLLQKLNGWYMEKEEPIYKTSCALTIHAIGRYSPDVLKNHAKEVLPLAFLGMHEIADEEKSEKEECNLWTEVWQENVPGSFGGIRLYLQELITITQKALQSQSWKMKAQGAIAMASIAKQTSSLVPPYLGMILTALLQGLAGRTWAGKEELLKAIACVVTACSAELEKSVPNQPSTNEILQAVLKECSKENVKYKIVAISCAADILKATKEDRFQEFSNIVIPLIKKNSLESSGVRTTKNEEENEKEKELQLEYLLGAFESLGKAWPRNAETQRCYRQELCKLMCERLKLSTWKVQLGVLQSMNAFFQGLMLLEEEHADPEALAEILLETCKSITYSLENKTYSSVRTEALSVIELLLKKLEESKQWECLTSECRVLLIESLATMEPDSRPELQEKAALLKKTLENLE |
| 分子量 | 82.72 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0368蛋白(USP32)的3篇文献示例，基于领域相关研究的典型方向整理：
1. **文献名称**：*USP32 regulates mitophagy through deubiquitination of Parkin*
**作者**：Wang Y. et al.
**摘要**：研究揭示了USP32作为去泛素化酶调控Parkin蛋白稳定性，影响线粒体自噬过程，通过重组人USP32蛋白体外实验证实其酶活性和对神经退行性疾病的潜在作用。
2. **文献名称**：*USP32 promotes cell proliferation and metastasis in triple-negative breast cancer*
**作者**：Li J. et al.
**摘要**：利用重组USP32蛋白进行功能实验，发现其通过去泛素化ERβ增强乳腺癌细胞的侵袭性，为USP32在肿瘤进展中的机制提供了新依据。
3. **文献名称**：*Structural basis of USP32 substrate specificity and inhibition*
**作者**：Chen L. et al.
**摘要**：通过重组表达纯化USP32催化结构域，结合晶体结构分析和生化实验，阐明了其底物识别特异性及小分子抑制剂的开发潜力。
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**说明**：以上文献为示例性整理，实际引用需查询具体数据库确认准确性。建议使用PubMed、Google Scholar等平台以“USP32”、“KIAA0368”及“recombinant protein”为关键词检索最新研究。
**Background of Recombinant Human KIAA0368 Protein**
The KIAA0368 gene, identified through large-scale cDNA sequencing projects, encodes a protein with largely uncharacterized functions, though bioinformatic analyses suggest potential roles in cellular processes such as protein interaction networks or enzymatic activity. As a member of the KIAA gene family, it is conserved across vertebrates, hinting at fundamental biological importance. The recombinant form of human KIAA0368 protein is engineered to enable functional and structural studies, typically expressed in mammalian or bacterial systems with purification tags (e.g., His-tag) for enhanced solubility and isolation.
Structurally, KIAA0368 contains conserved domains indicative of ATPase or kinase activity, though detailed mechanistic insights remain limited. Its recombinant version has been pivotal in investigating interactions with binding partners, enzymatic assays, and cellular localization. Research links KIAA0368 to neurological pathways and cancer progression, with emerging evidence suggesting involvement in cell cycle regulation or neuronal signaling. However, validation in disease models is ongoing.
Proteomic studies and interactome analyses position KIAA0368 within networks related to RNA processing and cytoskeletal dynamics. Recombinant production facilitates antibody development, structural biology (e.g., crystallography), and drug discovery pipelines targeting associated pathologies. Despite progress, its precise physiological role and therapeutic potential require further exploration using advanced omics and gene-editing tools. This protein exemplifies the broader challenge of translating genomic data into functional understanding for biomedical applications.
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