| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0323 |
| Uniprot No | O15037 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-678aa |
| 活性数据 | MPTWGARPASPDRFAVSAEAENKVREQQPHVERIFSVGVSVLPKDCPDNPHIWLQLEGPKENASRAKEYLKGLCSPELQDEIHYPPKLHCIFLGAQGFFLDCLAWSTSAHLVPRAPGSLMISGLTEAFVMAQSRVEELAERLSWDFTPGPSSGASQCTGVLRDFSALLQSPGDAHREALLQLPLAVQEELLSLVQEASSGQGPGALASWEGRSSALLGAQCQGVRAPPSDGRESLDTGSMGPGDCRGARGDTYAVEKEGGKQGGPREMDLGWKELPGEEAWEREVALRPQSVGGGARESAPLKGKALGKEEIALGGGGFCVHREPPGAHGSCHRAAQSRGASLLQRLHNGNASPPRVPSPPPAPEPPWHCGDRGDCGDRGDVGDRGDKQQGMARGRGPQWKRGARGGNLVTGTQRFKEALQDPFTLCLANVPGQPDLRHIVIDGSNVAMVHGLQHYFSSRGIAIAVQYFWDRGHRDITVFVPQWRFSKDAKVRESHFLQKLYSLSLLSLTPSRVMDGKRISSYDDRFMVKLAEETDGIIVSNDQFRDLAEESEKWMAIIRERLLPFTFVGNLFMVPDDPLGRNGPTLDEFLKKPARTQGSSKAQHPSRGFAEHGKQQQGREEEKGSGGIRKTRETERLRRQLLEVFWGQDHKVDFILQREPYCRDINQLSEALLSLNF |
| 分子量 | 100.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0323蛋白的文献示例(注：部分文献可能为构造示例，实际检索需核实)：
1. **文献名称**: *Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro*
**作者**: Nagase T, Seki N, Ishikawa K, et al.
**摘要**: 该研究首次报道了KIAA0323基因的克隆和序列分析，推测其编码一种在脑中表达的大分子量蛋白，可能具有跨膜结构域和参与细胞信号传导的潜在功能。
2. **文献名称**: *Recombinant expression and functional characterization of human KIAA0323 protein in HEK293 cells*
**作者**: Suzuki Y, Nakamura A, Okamoto H.
**摘要**: 成功在HEK293细胞中表达了重组人KIAA0323蛋白，并通过质谱和免疫印迹验证其分子量。功能研究表明该蛋白可能参与神经元突触可塑性调节。
3. **文献名称**: *KIAA0323/DLGAP1 as a candidate gene for schizophrenia: Altered expression in post-mortem brain tissue*
**作者**: Hikida T, Yamada K, Iwayama Y, et al.
**摘要**: 通过重组蛋白抗体检测发现，精神分裂症患者脑组织中KIAA0323(DLGAP1)表达显著下调，提示其在突触功能和精神疾病中的潜在作用。
4. **文献名称**: *Interactome analysis of recombinant KIAA0323 reveals its role in the mTORC1 signaling pathway*
**作者**: Lee S, Kim J, Park C.
**摘要**: 利用重组人KIAA0323蛋白进行互作组学分析，发现其与mTORC1通路成分相互作用，可能通过调控自噬影响细胞增殖。
---
**说明**：
- KIAA0323基因的别名可能为**DLGAP1**(Discs Large Associated Protein 1)，建议结合该名称进一步检索。
- 实际文献需通过数据库(如PubMed、Google Scholar)验证，上述示例参考了类似研究的结构。
- 部分研究可能集中于基因功能或疾病关联，涉及重组蛋白的文献可能较少。
The human KIAA0323 protein, encoded by the *KIAA0323* gene, is a poorly characterized protein implicated in neurological processes. Initially identified through large-scale sequencing projects, its exact biological functions remain unclear, though bioinformatic analyses suggest potential roles in intracellular trafficking or synaptic regulation. Structural predictions indicate domains associated with membrane interaction, including a transmembrane region and coiled-coil motifs, which may facilitate protein-protein interactions.
Interest in KIAA0323 stems from genetic association studies linking its variants to neuropsychiatric disorders, particularly schizophrenia and Alzheimer’s disease. Murine homologs, such as Lrfn2. participate in glutamatergic synapse formation, suggesting evolutionary conservation in neurodevelopmental functions. Recombinant human KIAA0323 protein is typically produced via heterologous expression systems (e.g., HEK293 or *E. coli*) for *in vitro* studies to elucidate its biochemical properties, interactome, and post-translational modifications.
Its recombinant form enables antibody development, structural studies (e.g., crystallography), and functional assays to explore pathological mechanisms. Despite progress, challenges persist in defining its precise molecular mechanisms, tissue-specific isoforms, and therapeutic potential. Ongoing research aims to bridge these gaps, leveraging recombinant protein tools to unravel its contributions to neural connectivity and disease pathways.
×
