| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0317 |
| Uniprot No | O15033 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-789aa |
| 活性数据 | MFYVIGGITVSVVAFFFTIKFLFELAARVVSFLQNEDRERRGDRTIYDYVRGNYLDPRSCKVSWDWKDPYEVGHSMAFRVHLFYKNGQPFPAHRPVGLRVHISHVELAVEIPVNQEVLQEPNSNVVKVAFTVRKAGRYEITVKLGGLNVAYSPYYKIFQPGMVVPSKTKIVCHFSTLVLTCGQPHTLQIVPRDEYDNPTNNSMSLRDEHNYTLSIHELGPQEEESTGVSFEKSVTSNRQTFQVFLRLTLHSRGCFHACISYQNQPINNGEFDIIVLSEDEKNIVERNVSTSGVSIYFEAYLYNATNCSSTPWHLPPMHMTSSQRRPSTAVDEEDEDSPSECHTPEKVKKPKKVYCYVSPKQFSVKEFYLKIIHWRLYTFRVCPGTKFSYLGPDPVHKLLTLVVDDGIQPPVELSCKERNILAATFIRSLHKNIGGSETFQDKVNFFQRELRQVHMKRPHSKVTLKVSRHALLESSLKATRNFSISDWSKNFEVVFQDEEALDWGGPRREWFELICKALFDTTNQLFTRFSDNNQALVHPNPNRPAHLRLKMYEFAGRLVGKCLYESSLGGAYKQLVRARFTRSFLAQIIGLRMHYKYFETDDPEFYKSKVCFILNNDMSEMELVFAEEKYNKSGQLDKVVELMTGGAQTPVTNANKIFYLNLLAQYRLASQVKEEVEHFLKGLNELVPENLLAIFDENELELLMCGTGDISVSDFKAHAVVVGGSWHFREKVMRWFWTVVSSLTQEELARLLQFTTGSSQLPPGGFAALCPSFQIIAAPTHSTLPTAHT |
| 分子量 | 116.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0317蛋白的3篇虚构参考文献示例(注：以下内容为模拟创作，实际文献需通过学术数据库检索确认)：
1. **文献名称**: "Cloning and Expression of Recombinant Human KIAA0317 Protein in E. coli"
**作者**: Zhang L, et al.
**摘要**: 本研究成功克隆了人KIAA0317基因的全长序列，并通过大肠杆菌表达系统实现了重组蛋白的体外可溶性表达。纯化后的蛋白经免疫印迹验证，为后续功能研究奠定了基础。
2. **文献名称**: "KIAA0317 Regulates Neuronal Cell Adhesion via Integrin Signaling Pathways"
**作者**: Tanaka K, et al.
**摘要**: 通过体外细胞实验发现，重组人KIAA0317蛋白能与整合素β1亚基结合，影响神经元细胞的黏附和迁移能力，提示其在神经系统发育中的潜在作用。
3. **文献名称**: "Proteomic Analysis of KIAA0317-Interacting Proteins in Colorectal Cancer"
**作者**: Wei Y, et al.
**摘要**: 本研究利用质谱技术筛选出与重组KIAA0317蛋白相互作用的多个候选蛋白(如RhoGTP酶家族成员)，并发现其在结直肠癌组织中高表达，可能参与肿瘤侵袭转移调控。
---
建议通过PubMed或Web of Science等平台检索关键词“recombinant human KIAA0317”或“KIAA0317 protein function”获取真实文献。
The recombinant human KIAA0317 protein is derived from the KIAA0317 gene, initially identified through large-scale sequencing efforts in the human genome project. This gene, located on chromosome 22 (22q13.2), encodes a transmembrane protein predominantly expressed in the brain, particularly in neurons and glial cells. While its precise biological function remains under investigation, KIAA0317 is hypothesized to play roles in neurodevelopment, cellular adhesion, and intracellular signaling. Structural analysis reveals multiple conserved domains, including a leucine-rich repeat (LRR) region and immunoglobulin-like folds, suggesting potential involvement in protein-protein interactions or extracellular matrix communication.
Recombinant KIAA0317 protein is typically produced using in vitro expression systems (e.g., mammalian cells or E. coli) to enable functional studies. Its recombinant form has become crucial for investigating molecular mechanisms in neurological disorders, as altered KIAA0317 expression has been tentatively linked to neurodevelopmental conditions such as autism spectrum disorders and schizophrenia-related phenotypes in preclinical models. Current research focuses on elucidating its interaction networks, particularly with synaptic proteins and components of Wnt signaling pathways. The development of recombinant KIAA0317 has accelerated antibody production, structural biology studies, and therapeutic target validation, though full characterization of its physiological and pathological significance requires further investigation.
×
