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Recombinant Human KCTD18 Protein

  • 中文名: 重组人KCTD18蛋白
  • 别    名: KCTD18; BTB/POZ domain-containing protein KCTD18
货号: PA2000-8608
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点KCTD18
Uniprot NoQ6PI47
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-426aa
活性数据MEGHKAEEEVLDVLRLNVGGCIYTARRESLCRFKDSMLASMFSGRFPLKTDESGACVIDRDGRLFKYLLDYLHGEVQIPTDEQTRIALQEEADYFGIPYPYSLSDHLANEMETYSLRSNIELKKALTDFCDSYGLVCNKPTVWVLHYLNTSGASCESRIIGVYATKTDGTDAIEKQLGGRIHSKGIFKREAGNNVQYIWSYYSVAELKKMMDAFDAWEGKGVSYWRVPHELIECWTLEERPLLGSLRHMAPIRKRRLITFNEADESVNYKTGPKPVRFLGPSTSTQIKVKNSASVTVSPASAIQTSAGATANRFQSGSRRKAAQRSAPSRATALVGTGAPGHPQASPGAASAENGGTHLPPAKVLLSDKKPTPQRVIKLKRTPLCATAPCLPSPTATRQANSLKPLPGEAARALGVRTENGKNKGN
分子量73.1 kDa
蛋白标签GST-tag at N-terminal
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于重组人KCTD18蛋白的示例参考文献(注意:以下为模拟内容,实际文献请通过专业数据库查询):

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1. **文献名称**:*Structural and Functional Characterization of Recombinant Human KCTD18 Protein*

**作者**:Zhang Y et al.

**摘要**:本研究成功在大肠杆菌中表达并纯化了重组人KCTD18蛋白,通过X射线晶体学解析其三维结构,发现其C端BTB结构域可能与泛素连接酶复合物相互作用,提示其在蛋白降解途径中的潜在功能。

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2. **文献名称**:*KCTD18 Regulates Neuronal Differentiation via Interaction with Notch Signaling Components*

**作者**:Wang L et al.

**摘要**:利用重组KCTD18蛋白进行体外实验,发现其通过与Notch受体胞内段的结合,抑制下游信号通路,影响神经干细胞的分化进程,为研究神经发育疾病提供了新方向。

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3. **文献名称**:*Expression and Purification of Recombinant KCTD18 in Mammalian Cells for Interaction Studies*

**作者**:Kim S et al.

**摘要**:开发了一种在HEK293细胞中高效表达重组KCTD18的方法,并通过免疫共沉淀实验证实其与Cul3蛋白的结合,表明其在Cullin-RING E3泛素连接酶复合物组装中的作用。

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4. **文献名称**:*KCTD18 Modulates Potassium Channel Activity in Cardiac Myocytes*

**作者**:Garcia-Rojas M et al.

**摘要**:重组KCTD18蛋白被证明与电压门控钾通道Kv4.2结合,通过调节通道失活动力学影响心肌细胞动作电位持续时间,可能参与心律失常的病理机制。

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**建议**:可通过PubMed、Web of Science等平台,以“recombinant KCTD18”、“KCTD18 expression”或“KCTD18 functional study”为关键词检索最新文献。


背景信息

Recombinant human KCTD18 (Potassium Channel Tetramerization Domain Containing 18) protein is a scientifically engineered form of the KCTD18 protein, designed to facilitate molecular and functional studies. Belonging to the KCTD family, it shares a conserved N-terminal BTB (Bric-a-brac, Tramtrack, Broad Complex) domain, which mediates protein-protein interactions and participates in ubiquitination-related processes by associating with Cullin3-based E3 ubiquitin ligase complexes. While the full physiological role of KCTD18 remains unclear, emerging evidence suggests its involvement in cellular proliferation, differentiation, and apoptosis regulation, potentially linking it to cancer biology, neurodevelopmental disorders, or immune modulation. The recombinant version, typically expressed in bacterial or mammalian systems, enables researchers to explore its structure, binding partners, and post-translational modifications without interference from endogenous cellular components. Its study is particularly relevant for deciphering mechanisms underlying ubiquitin-dependent protein degradation pathways and identifying novel therapeutic targets. Current research focuses on characterizing its tissue-specific expression patterns, interactome networks, and mutational impacts in disease contexts. However, functional data remain sparse compared to other KCTD members, highlighting the need for further investigation using recombinant tools to clarify its biological significance and translational potential in precision medicine.


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