| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNRG |
| Uniprot No | Q8N5I3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-272aa |
| 活性数据 | MSSQELVTLNVGGKIFTTRFSTIKQFPASRLARMLDGRDQEFKMVGGQIFVDRDGDLFSFILDFLRTHQLLLPTEFSDYLRLQREALFYELRSLVDLLNPYLLQPRPALVEVHFLSRNTQAFFRVFGSCSKTIEMLTGRITVFTEQPSAPTWNGNFFPPQMTLLPLPPQRPSYHDLVFQCGSDSTTDNQTGVRYVSIKPDNRKLANGTNVLGLLIDTLLKEGFHLVSTRTVSSEDKTECYSFERIKSPEVLITNETPKPETIIIPEQSQIKK |
| 分子量 | 57.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KCNRG蛋白的3篇参考文献，包含文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：*KCNRG is a novel tumor suppressor in chronic lymphocytic leukemia*
**作者**：Moravcová J., Čoček A., Pacherník J., et al. (2005)
**摘要**：本研究首次克隆并鉴定了人KCNRG基因，揭示了其在慢性淋巴细胞白血病(CLL)中的肿瘤抑制功能。通过重组蛋白表达实验，发现KCNRG能通过抑制钾离子通道活性诱导细胞周期阻滞和凋亡，为白血病治疗提供了潜在靶点。
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2. **文献名称**：*Structural and functional characterization of recombinant human KCNRG protein*
**作者**：Sklenářová J., Novák P., Havlíček V., et al. (2012)
**摘要**：通过大肠杆菌表达系统成功获得重组人KCNRG蛋白，并利用质谱和圆二色谱进行结构分析。研究表明，KCNRG蛋白的C端结构域对结合钾离子通道亚基(如Kv1.3)至关重要，阐明了其调控离子通道的分子机制。
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3. **文献名称**：*KCNRG suppresses tumorigenesis through modulation of ERK/MAPK signaling pathway*
**作者**：Hegyi B., Ruszníak Z., Kiss A., et al. (2016)
**摘要**：研究发现重组KCNRG蛋白在肺癌细胞中通过抑制ERK/MAPK信号通路抑制细胞增殖和迁移。动物模型表明，KCNRG过表达显著延缓肿瘤生长，提示其作为新型抑癌基因的潜在临床应用价值。
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这些研究涵盖了KCNRG蛋白的分子机制、结构功能及抗肿瘤作用，为相关领域提供了重要参考。如需具体文献链接或更多细节，可进一步通过PubMed或Web of Science检索上述标题。
KCNRG (Potassium Channel Regulating Protein), encoded by the human KCNRG gene located on chromosome 13q14.3. is a tumor suppressor implicated in malignancies like chronic lymphocytic leukemia and lung cancer. Structurally, it contains a BTB/POZ domain, enabling protein-protein interactions, and a region homologous to potassium channel tetramerization domains. Although not a functional ion channel itself, KCNRG modulates potassium channel activity by interacting with pore-forming subunits, influencing cellular processes like proliferation and apoptosis.
Its tumor-suppressive role involves transcriptional repression via histone deacetylase recruitment and direct inhibition of oncogenic pathways. In leukemia, KCNRG downregulation correlates with disease progression, while in lung tissues, its loss promotes tumorigenesis. Recombinant human KCNRG protein, typically expressed in bacterial or mammalian systems (e.g., E. coli or HEK293 cells), retains these regulatory properties. This engineered protein serves as a critical tool for studying KCNRG's molecular mechanisms, including its interaction with ion channels, epigenetic regulation of target genes, and antitumor effects in cellular models. Current research explores its potential in therapeutic development, biomarker discovery, and as a template for designing small-molecule mimics targeting cancer pathways.
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