| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNMB4 |
| Uniprot No | Q86W47 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 活性数据 | MAKLRVAYEYTEAEDKSIRLGLFLIISGVVSLFIFGFCWLSPALQDLQATEANCTVLSVQQIGEVFECTFTCGADCRGTSQYPCVQVYVNNSESNSRALLHSDEHQLLTNPKCSYIPPCKRENQKNLESVMNWQQYWKDEIGSQPFTCYFNQHQRPDDVLLHRTHDEIVLLHCFLWPLVTFVVGVLIVVLTICAKSLAVKAEAMKKRKFS |
| 分子量 | 48.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KCNMB4蛋白的模拟参考文献示例(注：以下内容为假设性构建，真实文献需通过学术数据库查询)：
---
1. **文献名称**：**"Functional Modulation of BK Channels by the β4 Subunit in Neuronal Excitability"**
**作者**：Brenner, R., et al.
**摘要**：该研究探讨了重组人KCNMB4蛋白(BK通道β4亚基)在调节神经元钙激活钾通道中的作用，发现其通过降低通道的钙敏感性抑制高频动作电位，揭示了其在控制神经元兴奋性中的关键机制。
2. **文献名称**：**"Expression and Electrophysiological Characterization of Recombinant KCNMB4 in Mammalian Cells"**
**作者**：Shipston, M.J., et al.
**摘要**：文章报道了在HEK293细胞中重组表达人KCNMB4蛋白的方法，证明其与α亚基共表达可显著延长BK通道的激活时间，并增强其在睾丸组织中的生理功能。
3. **文献名称**：**"Structural Insights into KCNMB4-Mediated Modulation of BK Channel Voltage Sensing"**
**作者**：Magleby, K.L., et al.
**摘要**：通过冷冻电镜和功能实验，研究发现KCNMB4亚基与BK通道α亚基的结合改变了电压传感器结构，降低电压依赖性，为设计靶向药物提供了结构基础。
4. **文献名称**：**"KCNMB4 Polymorphisms and Their Association with Epilepsy Susceptibility"**
**作者**：Gonzalez-Perez, V., et al.
**摘要**：此研究通过表达携带特定突变的重组KCNMB4蛋白，发现其功能异常与BK通道活动受损相关，提示KCNMB4基因变异可能增加癫痫遗传风险。
---
如需真实文献，建议访问 **PubMed**(https://pubmed.ncbi.nlm.nih.gov)或 **Google Scholar**，搜索关键词如 **"recombinant KCNMB4"、"BK channel beta4 subunit"**，并筛选近年高被引研究。
The human KCNMB4 protein, a member of the potassium calcium-activated channel subfamily M beta (KCNMB), serves as a regulatory β4-subunit for large-conductance Ca²⁺-activated K⁺ (BK or KCa1.1) channels. These channels play critical roles in modulating cellular excitability, vascular tone, and neurotransmitter release by integrating Ca²⁺ and voltage signals. The KCNMB4 subunit specifically influences BK channel kinetics, reducing their calcium sensitivity and slowing activation, which fine-tunes cellular responses in tissues where it is expressed, such as the central nervous system, smooth muscle, and endocrine cells.
Recombinant human KCNMB4 protein is typically produced using expression systems like HEK293 or *E. coli* with affinity tags (e.g., His-tag) to facilitate purification. Its recombinant form enables structural and functional studies, particularly in elucidating mechanisms underlying BK channel diversity and disease-associated variants. Dysregulation of KCNMB4 has been implicated in neurological disorders, hypertension, and cancer, making it a potential therapeutic target. Researchers employ this protein in electrophysiological assays, drug screening, and protein interaction studies to explore its role in channelopathies and develop modulators. Its study also aids in understanding tissue-specific regulation of BK channels, offering insights into precision medicine approaches for cardiovascular and neurological conditions.
×
