| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNK12 |
| Uniprot No | Q9HB15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430aa |
| 活性数据 | MSSRSPRPPPRRSRRRLPRPSCCCCCCRRSHLNEDTGRFVLLAALIGLYLVAGATVFSALESPGEAEARARWGATLRNFSAAHGVAEPELRAFLRHYEAALAAGVRADALRPRWDFPGAFYFVGTVVSTIGFGMTTPATVGGKAFLIAYGLFGCAGTILFFNLFLERIISLLAFIMRACRERQLRRSGLLPATFRRGSALSEADSLAGWKPSVYHVLLILGLFAVLLSCCASAMYTSVEGWDYVDSLYFCFVTFSTIGFGDLVSSQHAAYRNQGLYRLGNFLFILLGVCCIYSLFNVISILIKQVLNWMLRKLSCRCCARCCPAPGAPLARRNAITPGSRLRRRLAALGADPAARDSDAEGRRLSGELISMRDLTASNKVSLALLQKQLSETANGYPRSVCVNTRQNGFSGGVGALGIMNNRLAETSASR |
| 分子量 | 46.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KCNK12蛋白的参考文献示例(部分内容为模拟创作，实际文献需通过学术数据库查询)：
1. **文献名称**: "Functional Characterization of Recombinant Human KCNK12 Potassium Channel in Xenopus Oocytes"
**作者**: Zhang Y, et al.
**摘要**: 本研究在非洲爪蟾卵母细胞中成功表达重组人KCNK12蛋白，证实其形成pH敏感的钾电流，并对多种双孔钾通道调节剂(如异丙酚)有响应，揭示了其在中枢神经系统中的潜在作用机制。
2. **文献名称**: "Cryo-EM Structure of the Human KCNK12 Channel Reveals a Unique Leak Current Regulation Mechanism"
**作者**: Li H, Wang J.
**摘要**: 通过冷冻电镜技术解析重组KCNK12蛋白的高分辨率结构，发现其C端结构域通过动态构象变化调节离子通透性，为开发靶向KCNK12的神经疾病药物提供结构基础。
3. **文献名称**: "Role of KCNK12 in Cancer Cell Proliferation: Insights from Recombinant Protein Knockdown Models"
**作者**: Chen L, et al.
**摘要**: 利用重组KCNK12蛋白构建的体外模型表明，该通道通过调控线粒体膜电位影响肺癌细胞增殖，提示其可能成为肿瘤治疗的新靶点。
4. **文献名称**: "Development of a High-Throughput Screening Assay for KCNK12 Modulators Using Fluorescent Probes"
**作者**: Gupta S, et al.
**摘要**: 开发基于重组KCNK12蛋白的荧光标记高通量筛选平台，发现多个小分子化合物可调节其活性，为治疗癫痫和抑郁症提供先导化合物。
如需真实文献，建议通过PubMed或Web of Science检索关键词“recombinant KCNK12”或“hKCNK12 expression”获取最新研究。
Recombinant human KCNK12 protein is a product of the KCNK12 gene, which encodes a member of the two-pore domain potassium (K2P) channel family. Also known as THIK-2 (tandem pore domain halothane-inhibited K+ channel 2), KCNK12 plays a critical role in maintaining cellular resting membrane potential and regulating electrical excitability. As a background leak potassium channel, it contributes to K+ efflux, stabilizing membrane voltage and modulating neuronal or muscular activity. Structurally, K2P channels like KCNK12 feature two pore-forming domains and four transmembrane segments, enabling voltage-independent potassium conduction. KCNK12 is distinguished by its sensitivity to extracellular pH, mechanical stress, and specific pharmacological agents. Its expression has been documented in the brain, heart, and smooth muscle tissues, suggesting roles in neuroprotection, cardiac rhythm regulation, and vascular tone. Recombinant KCNK12 protein, typically expressed in mammalian or insect cell systems, facilitates in vitro studies on channel gating mechanisms, drug interactions, and disease correlations. Dysregulation of KCNK12 has been implicated in pathologies such as arrhythmias, hypertension, and neurological disorders, though its precise physiological and pathological roles remain under investigation. Current research focuses on developing selective modulators to probe its therapeutic potential. The recombinant protein serves as a vital tool for elucidating structure-function relationships and screening compounds targeting K2P channels.
×
