| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNAB3 |
| Uniprot No | O43448 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-404aa |
| 活性数据 | MQVSIACTEQ NLRSRSSEDR LCGPRPGPGG GNGGPAGGGH GNPPGGGGSG PKARAALVPR PPAPAGALRE STGRGTGMKY RNLGKSGLRV SCLGLGTWVT FGSQISDETA EDVLTVAYEH GVNLFDTAEV YAAGKAERTL GNILKSKGWR RSSYVITTKI FWGGQAETER GLSRKHIIEG LRGSLERLQL GYVDIVFANR SDPNCPMEEI VRAMTYVINQ GLALYWGTSR WGAAEIMEAY SMARQFNLIP PVCEQAEHHL FQREKVEMQL PELYHKIGVG SVTWYPLACG LITSKYDGRV PDTCRASIKG YQWLKDKVQS EDGKKQQAKV MDLLPVAHQL GCTVAQLAIA WCLRSEGVSS VLLGVSSAEQ LIEHLGALQV LSQLTPQTVM EIDGLLGNKP HSKK |
| 分子量 | 43.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KCNAB3蛋白的模拟参考文献示例(注：实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Functional Modulation of Kv1.5 Channels by Recombinant Human KCNAB3 Protein*
**作者**：Li, X.; Zhang, Y.; Wang, H.
**摘要**：本研究利用重组表达技术获得了高纯度的人KCNAB3蛋白，并发现其通过调控Kv1.5钾通道的门控动力学增强通道的快速失活特性，揭示了其在心脏电生理中的潜在作用。
2. **文献名称**：*Structural Insights into the Regulatory Role of KCNAB3 in Potassium Channels*
**作者**：Garcia-Calvo, M.; Kaczorowski, G.J.
**摘要**：通过冷冻电镜解析重组人KCNAB3蛋白与Kv1.3通道复合物的结构，阐明其通过C端结构域与通道α亚基互作，调节通道的电压敏感性和细胞膜定位。
3. **文献名称**：*KCNAB3 Gene Variants and Recombinant Protein Expression in Neurological Disorders*
**作者**：Chen, L.; Wei, F.
**摘要**：研究发现特定KCNAB3突变体导致重组蛋白稳定性下降，并通过体外实验表明其功能缺陷可能与癫痫等神经通道病的发病机制相关。
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**备注**：
- 上述文献为示例性内容，实际研究中请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 等平台，以关键词“recombinant KCNAB3”、“KCNAB3 protein”等检索最新文献。
- 建议关注《Journal of Biological Chemistry》《Nature Structural & Molecular Biology》等期刊的相关研究。
Recombinant human KCNAB3 protein is a genetically engineered form of the potassium voltage-gated channel subfamily A regulatory beta subunit 3. designed to facilitate studies on its structure and function. KCNAB3. encoded by the KCNAB3 gene on chromosome 17. belongs to the Kvβ family of proteins that modulate the activity of voltage-gated potassium (Kv) channels. It binds to the α-subunits of Kv1 channels (e.g., Kv1.4. Kv1.5), influencing channel gating kinetics, trafficking, and stability. This regulation is critical for controlling cellular excitability, action potential duration, and neurotransmitter release in neurons, as well as cardiac repolarization. Dysregulation of KCNAB3 has been linked to neurological disorders and cardiac arrhythmias.
The recombinant protein is typically expressed in bacterial or mammalian systems, purified via affinity chromatography, and validated for functional assays. It serves as a tool to study Kv channel modulation, screen potential therapeutic agents targeting potassium channelopathies, and investigate molecular mechanisms underlying diseases like epilepsy or Brugada syndrome. Alternative splicing generates multiple KCNAB3 isoforms, adding complexity to its functional roles. By providing a controlled, high-purity source of KCNAB3. the recombinant protein enables precise exploration of its interaction networks and disease-associated mutations, aiding both basic research and drug discovery.
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