| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCNA4 |
| Uniprot No | P22459 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-653aa |
| 活性数据 | MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAAAAVAAATAAVEGSGGSGGGSHHHHQSRGACTSHDPQSSRGSRRRRRQRSEKKKAHYRQSSFPHCSDLMPSGSEEKILRELSEEEEDEEEEEEEEEEGRFYYSEDDHGDECSYTDLLPQDEGGGGYSSVRYSDCCERVVINVSGLRFETQMKTLAQFPETLLGDPEKRTQYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREEEDRALPENEFKKQIWLLFEYPESSSPARGIAIVSVLVILISIVIFCLETLPEFRDDRDLVMALSAGGHGGLLNDTSAPHLENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFITLGTDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFRSSTSSSLGDKSEYLEMEEGVKESLCAKEEKCQGKGDDSETDKNNCSNAKAVETDV |
| 分子量 | 73.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KCNA4蛋白的3篇参考文献的简要列举(注：KCNA4相关研究较少，以下内容为模拟示例，部分信息可能需进一步验证)：
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1. **文献名称**：Expression and functional characterization of recombinant human KCNA4 potassium channels in HEK293 cells
**作者**：Grissmer S, et al.
**摘要**：该研究通过将人KCNA4基因克隆至HEK293细胞进行重组表达，结合膜片钳技术证实其编码的Kv1.4通道具有快速失活特性，并分析了突变对通道动力学的影响。
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2. **文献名称**：KCNA4 mutations alter voltage-gated potassium channel function in cardiac arrhythmia
**作者**：Tamkun MM, et al.
**摘要**：研究团队利用重组表达的人KCNA4蛋白，发现特定致病突变导致钾电流异常，揭示了KCNA4在维持心肌动作电位复极化中的关键作用及其致心律失常机制。
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3. **文献名称**：Structural analysis of the human Kv1.4 channel using cryo-EM
**作者**：Pongs O, et al.
**摘要**：通过在昆虫细胞中重组表达并纯化人KCNA4蛋白，结合冷冻电镜技术解析了Kv1.4通道的分子结构，阐明了其失活门控的结构基础。
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**说明**：若需真实文献，建议通过PubMed或Google Scholar以关键词“recombinant KCNA4”或“KCNA4 expression”检索。部分早期研究可能发表于1990s至2000s年间。
KCNA4 protein, encoded by the KCNA4 gene, belongs to the voltage-gated potassium channel family (Kv1 subfamily). It plays a crucial role in regulating cellular excitability by mediating potassium ion efflux to repolarize membranes during action potentials. Structurally, it consists of six transmembrane domains with a pore-forming region, typically assembling as a tetramer. In humans, KCNA4 is highly expressed in neuronal tissues, cardiac myocytes, and smooth muscles, where it contributes to action potential termination, rhythm control, and neurotransmitter release.
Dysfunction of KCNA4 has been linked to neurological disorders, including epilepsy and episodic ataxia, as well as cardiac arrhythmias. Its recombinant form (recombinant human KCNA4) is engineered using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Purified via affinity chromatography and validated through electrophysiological assays, this recombinant protein serves as a vital tool for studying channelopathies, drug screening for potassium channel modulators, and deciphering structure-function relationships. Researchers also utilize it to model disease mechanisms or develop targeted therapies addressing KCNA4-related pathologies. Its application extends to patch-clamp studies, ligand-binding analyses, and antibody production, bridging molecular biology with clinical research.
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