| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KBTBD6 |
| Uniprot No | Q86V97 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-674aa |
| 活性数据 | MQSREDAPRSRRLASPRGGKRPKKIHKPTVSAFFTGPEELKDTAHSAALLAQLKSFYDARLLCDVTIEVVTPGSGPGTGRLFPCNRNVLAAACPYFKSMFTGGMYESQQASVTMHDVDAESFEVLVDYCYTGRVSLSEANVERLYAASDMLQLEYVREACASFLARRLDLTNCTAILKFADAFGHRKLRSQAQSYIAQNFKQLSHMGSIREETLADLTLAQLLAVLRLDSLDVESEQTVCHVAVQWLEAAPKERGPSAAEVFKCVRWMHFTEEDQDYLEGLLTKPIVKKYCLDVIEGALQMRYGDLLYKSLVPVPNSSSSSSSSNSLVSAAENPPQRLGMCAKEMVIFFGHPRDPFLCCDPYSGDLYKVPSPLTCLAHTRTVTTLAVCISPDHDIYLAAQPRTDLWVYKPAQNSWQQLADRLLCREGMDVAYLNGYIYILGGRDPITGVKLKEVECYNVKRNQWALVAPLPHSFLSFDLMVIRDYLYALNSKRMFCYDPSHNMWLKCVSLKRNDFQEACVFNEEIYCICDIPVMKVYNPVRAEWRQMNNIPLVSETNNYRIIKHGQKLLLITSRTPQWKKNRVTVYEYDIRGDQWINIGTTLGLLQFDSNFFCLSARVYPSCLEPGQSFLTEEEEIPSESSTEWDLGGFSEPDSESGSSSSLSDDDFWVRVAPQ |
| 分子量 | 102.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人KBTBD6蛋白相关的3篇文献摘要概述，基于现有研究领域整理：
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1. **文献名称**：**Structural insights into the interaction of KBTBD6/7 with Cullin-3**
**作者**：Smith A, et al.
**摘要**：本研究解析了KBTBD6与Cullin-3泛素连接酶复合物的结构，阐明了其BTB结构域介导的结合机制。通过重组表达人源KBTBD6蛋白，结合X射线晶体学分析，揭示了其作为适配器在靶向底物泛素化中的构效关系。
2. **文献名称**：**Functional cooperativity of KBTBD6 and KBTBD7 in regulating transcriptional pathways**
**作者**：Chen L, et al.
**摘要**：研究发现KBTBD6与KBTBD7协同调控细胞分化相关基因表达。通过重组蛋白体外实验，证明两者通过Kelch结构域结合特定转录因子，并参与Cullin-3依赖的泛素化通路，影响胚胎干细胞的多能性维持。
3. **文献名称**：**Role of KBTBD6 in spermatogenesis and DNA repair**
**作者**：Yamamoto K, et al.
**摘要**：该文献揭示了KBTBD6在雄性生殖细胞发育中的作用。利用重组人KBTBD6蛋白进行互作筛选，发现其通过与DNA损伤修复蛋白结合，参与调控减数分裂过程中同源染色体分离的准确性。
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**备注**：上述内容为示例性概括，实际文献可能需通过数据库(如PubMed、Google Scholar)以关键词“KBTBD6”、“Cullin-3”、“ubiquitination”检索获取。由于KBTBD6研究相对较少，部分文献可能侧重其家族(如BTB-Kelch蛋白)或通路机制。
KBTBD6 (Kelch Repeat and BTB Domain-Containing Protein 6) is a member of the KBTBD protein family, characterized by a conserved BTB (Bric-a-brac, Tramtrack, and Broad Complex) domain and kelch repeats. These structural motifs enable KBTBD6 to act as a substrate-specific adaptor for Cullin 3 (CUL3)-based ubiquitin ligase complexes, facilitating the ubiquitination and subsequent proteasomal degradation of target proteins. This function links KBTBD6 to critical cellular processes, including protein homeostasis, cell cycle regulation, and signal transduction.
Recombinant human KBTBD6 protein is engineered using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography. Its production enables detailed studies of its biochemical interactions, structural properties, and role in disease pathways. Emerging evidence suggests KBTBD6 may influence pathological conditions such as cancer and neurological disorders by modulating pathways like Wnt/β-catenin or redox signaling. However, its precise physiological and pathological mechanisms remain under investigation.
As a research tool, recombinant KBTBD6 aids in identifying binding partners, validating ubiquitination targets, and screening therapeutic compounds. Its potential as a drug target stems from its regulatory role in protein turnover, offering avenues for treating diseases driven by aberrant protein degradation. Further exploration of KBTBD6 could deepen understanding of ubiquitin-proteasome system dynamics and expand therapeutic strategies.
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