| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KBTBD4 |
| Uniprot No | Q9NVX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518aa |
| 活性数据 | MESPEEPGAS MDENYFVNYT FKDRSHSGRV AQGIMKLCLE EELFADVTIS VEGREFQLHR LVLSAQSCFF RSMFTSNLKE AHNRVIVLQD VSESVFQLLV DYIYHGTVKL RAEELQEIYE VSDMYQLTSL FEECSRFLAR TVQVGNCLQV MWLADRHSDP ELYTAAKHCA KTHLAQLQNT EEFLHLPHRL LTDIISDGVP CSQNPTEAIE AWINFNKEER EAFAESLRTS LKEIGENVHI YLIGKESSRT HSLAVSLHCA EDDSISVSGQ NSLCHQITAA CKHGGDLYVV GGSIPRRMWK CNNATVDWEW CAPLPRDRLQ HTLVSVPGKD AIYSLGGKTL QDTLSNAVIY YRVGDNVWTE TTQLEVAVSG AAGANLNGII YLLGGEENDL DFFTKPSRLI QCFDTETDKC HVKPYVLPFA GRMHAAVHKD LVFIVAEGDS LVCYNPLLDS FTRLCLPEAW SSAPSLWKIA SCNGSIYVFR DRYKKGDANT YKLDPATSAV TVTRGIKVLL TNLQFVLA |
| 分子量 | 59.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KBTBD4蛋白的3篇虚构参考文献(基于领域常见研究方向概括)：
1. **文献名称**：*Structural and Functional Analysis of Human KBTBD4 in Ubiquitin Ligase Complexes*
**作者**：Chen L. et al.
**摘要**：解析重组人KBTBD4蛋白的晶体结构，揭示其通过BTB结构域与Cullin3结合，调控底物泛素化的分子机制，证实其在神经发育相关通路中的潜在作用。
2. **文献名称**：*KBTBD4 Mutations Disrupt Notch Signaling in Neuroblastoma*
**作者**：Wang Y. et al.
**摘要**：利用重组KBTBD4蛋白研究其与Notch信号通路的相互作用，发现其在神经母细胞瘤中高频突变导致泛素化异常，促进肿瘤细胞增殖和耐药性。
3. **文献名称**：*Recombinant KBTBD4 Expression and Its Role in Neuronal Differentiation*
**作者**：Smith J. et al.
**摘要**：研究重组KBTBD4蛋白在体外神经元分化模型中的作用，证实其通过降解特定转录因子(如HES1)调控神经干细胞命运决定。
---
*注：以上文献及内容为概括性示例，实际研究中请查阅具体数据库(如PubMed)获取真实资料。*
Recombinant human KBTBD4 is a protein of interest in cellular regulation and disease research. KBTBD4 belongs to the Kelch repeat and BTB (Broad-Complex, Tramtrack, and Bric-a-brac) domain-containing protein family, characterized by its conserved Kelch repeats (mediating substrate recognition) and BTB domain (facilitating protein-protein interactions). It functions as an adaptor for Cullin3 (CUL3)-based E3 ubiquitin ligase complexes, targeting specific substrates for ubiquitination and subsequent proteasomal degradation. This post-translational modification mechanism is critical for maintaining cellular homeostasis, signaling, and stress responses.
KBTBD4 has been implicated in neurological processes, including neuronal differentiation and synaptic plasticity. Recent studies suggest its role in cancer, particularly gliomas, where genomic alterations in KBTBD4 correlate with dysregulated ubiquitination pathways and tumor progression. Its involvement in DNA damage response and chromatin remodeling further highlights its regulatory versatility.
Recombinant KBTBD4 is typically produced in bacterial or mammalian expression systems, enabling in vitro studies on substrate specificity, enzymatic activity, and structural features. Purification methods often involve affinity chromatography (e.g., His-tag or GST-tag systems) followed by functional validation. Research on recombinant KBTBD4 aids in deciphering its pathophysiological roles and exploring therapeutic strategies targeting ubiquitination pathways in neurodegenerative disorders or cancers. Ongoing investigations aim to map its interactome and clarify its context-dependent regulatory mechanisms.
×
