| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KATNAL2 |
| Uniprot No | Q8IYT4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538aa |
| 活性数据 | MELSYQTLKF THQAREACEM RTEARRKNLL ILISHYLTQE GYIDTANALE QETKLGLRRF EVCDNIDLET ILMEYESYYF VKFQKYPKIV KKSSDTAENN LPQRSRGKTR RMMNDSCQNL PKINQQRPRS KTTAGKTGDT KSLNKEHPNQ EVVDNTRLES ANFGLHISRI RKDSGEENAH PRRGQIIDFQ GLLTDAIKGA TSELALNTFD HNPDPSERLL KPLSAFIGMN SEMRELAAVV SRDIYLHNPN IKWNDIIGLD AAKQLVKEAV VYPIRYPQLF TGILSPWKGL LLYGPPGTGK TLLAKAVATE CKTTFFNISA STIVSKWRGD SEKLVRVLFE LARYHAPSTI FLDELESVMS QRGTASGGEH EGSLRMKTEL LVQMDGLARS EDLVFVLAAS NLPWELDCAM LRRLEKRILV DLPSREARQA MIYHWLPPVS KSRALELHTE LEYSVLSQET EGYSGSDIKL VCREAAMRPV RKIFDALENH QSESSDLPRI QLDIVTTADF LDVLTHTKPS AKNLAQRYSD WQREFESV |
| 分子量 | 61.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性示例，供参考。建议通过PubMed或Google Scholar检索实际文献：
1. **标题**：*Structural Insights into Human KATNAL2 Reveal Its Microtubule-Severing Mechanism*
**作者**：Smith J. et al. (2018)
**摘要**：通过冷冻电镜解析KATNAL2蛋白结构，阐明其依赖ATP的微管切割机制，揭示与驱动蛋白家族的其他成员的结构差异。
2. **标题**：*KATNAL2 Regulates Ciliogenesis via Tubulin Modification in Human Cells*
**作者**：Lee H. et al. (2019)
**摘要**：研究发现KATNAL2通过切割微管调控纤毛形成，敲低KATNAL2导致纤毛长度异常，提示其在细胞动力学中的关键作用。
3. **标题**：*Association of KATNAL2 Mutations with Neurodevelopmental Disorders*
**作者**：Garcia R. et al. (2020)
**摘要**：全外显子测序发现KATNAL2突变与儿童神经发育迟缓相关，暗示其在神经元迁移或突触形成中的潜在功能。
4. **标题**：*Optimized Expression and Purification of Recombinant Human KATNAL2 in E. coli*
**作者**：Chen L. et al. (2021)
**摘要**：报道KATNAL2在大肠杆菌中的重组表达策略，采用GST标签纯化并验证其体外微管切割活性，为功能研究提供方法学支持。
**注意**：以上为模拟示例，具体文献需检索学术数据库确认。实际研究中KATNAL2可能与纤毛疾病、不育症或神经退行性疾病相关。
**Background of Recombinant Human KATNAL2 Protein**
KATNAL2 (Katanin Catalytic Subunit A1 Like 2) is a member of the katanin family of microtubule-severing enzymes, which play critical roles in regulating microtubule dynamics by generating internal breaks within microtubules. This protein contains a conserved AAA+ ATPase domain, enabling ATP-dependent enzymatic activity, and is implicated in cellular processes such as mitosis, cilia formation, and neuronal development. KATNAL2 is structurally related to KATNA1 but exhibits distinct tissue-specific expression patterns and regulatory functions, particularly in ciliary disassembly and spermatogenesis.
Recombinant human KATNAL2 protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and functional integrity for experimental studies. Its production enables researchers to explore enzymatic kinetics, substrate interactions, and structural properties. Dysregulation of KATNAL2 has been linked to ciliopathies, infertility, and neurodevelopmental disorders, underscoring its biomedical relevance. Current research focuses on deciphering its role in microtubule remodeling mechanisms and potential therapeutic applications, such as targeting microtubule-associated pathologies. The development of recombinant KATNAL2 also facilitates drug screening and molecular tool design to dissect pathways involving microtubule regulation in health and disease.
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