Cat: PA2000-1773

Recombinant E.coli acpS Protein,His

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关键信息

  • 基因名

    acpS

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    acpS;dpj;Holo-[acyl-carrier-Protein] synthase

  • 种属

    E.coli

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P63471

  • 表达区间

    1-119aa

  • 氨基酸序列

    MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNPKRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSISHSGNYAQASVILEEEK

  • 分子量

    20.7 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

质检流程

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背景信息

ACP S (acyl carrier protein S) is a crucial component in the fatty acid biosynthesis pathway across various organisms, including bacteria, plants, and animals. As a fundamental player in the synthesis of fatty acids, ACP S is involved in the transport of acyl groups and interacts with various enzymes that facilitate the elongation and modification of fatty acid chains. Understanding the structure and function of ACP S is critical for elucidating lipid metabolism and cellular energy storage mechanisms. Recent studies have highlighted the significance of ACP S in the development of antibiotic resistance, as certain bacteria depend on fatty acids for membrane integrity and virulence. Additionally, recombinant ACP S proteins are being explored for potential applications in biotechnology, such as the production of biofuels and bio-based chemicals through engineered metabolic pathways. The recombination of ACP S allows researchers to investigate its biochemical properties, interaction with ligands, and its role in various metabolic processes. Furthermore, unraveling the mechanism of ACP S can lead to the design of novel inhibitors that target fatty acid synthesis in pathogenic bacteria, providing a promising avenue for developing new antimicrobial agents. Consequently, the study of recombinant ACP S not only deepens our understanding of fundamental biological processes but also holds significant implications for health and sustainability in the context of food security and energy resources.

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