| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JARID1D |
| Uniprot No | Q9BY66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 127-230aa |
| 活性数据 | AICKDRRWARVAQRLHYPPGKNIGSLLRSHYERIIYPYEMFQSGANHVQCNTHPFDNEVKDKEYKPHSIPLRQSVQPSKFSSYSRRAKRLQPDPEPTEEDIEKH |
| 分子量 | 37.07 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人JARID1D蛋白的参考文献示例(文献名为虚构示例，仅供参考)：
1. **"Functional characterization of recombinant human JARID1D in histone demethylation"**
*Authors: Smith A., et al.*
摘要：研究报道了重组人JARID1D蛋白的表达纯化及其对组蛋白H3K4me3/me2去甲基化活性的验证，阐明其在表观遗传调控中的作用。
2. **"Structural insights into JARID1D catalytic mechanism by cryo-EM"**
*Authors: Li X., et al.*
摘要：通过冷冻电镜解析重组人JARID1D蛋白的三维结构，揭示其底物结合域和去甲基化酶活性中心的分子机制。
3. **"JARID1D knockdown impairs spermatogenesis via aberrant chromatin remodeling"**
*Authors: Chen L., et al.*
摘要：利用重组JARID1D蛋白进行功能补偿实验，发现其在男性生殖细胞发育中通过调控靶基因染色质状态维持精子形成。
4. **"Development of a high-throughput assay for JARID1D inhibitors screening"**
*Authors: Park S., et al.*
摘要：基于重组人JARID1D蛋白构建体外酶活性检测体系，用于筛选靶向其去甲基化酶活性的抗癌化合物。
注：以上文献名为示例，实际文献需通过PubMed或专业数据库(如Nature、Science、Cell子刊)检索真实发表内容。建议使用关键词"JARID1D/KDM5D recombinant protein"或"JARID1D function"查询近期论文。
**Background of Recombinant Human JARID1D Protein**
JARID1D (Jumonji/AT-rich interaction domain-containing protein 1D), also known as KDM5D, is a member of the JARID1 subfamily within the KDM5 histone demethylase group. It functions as a lysine-specific demethylase, targeting tri- and di-methylated histone H3 lysine 4 (H3K4me3/me2), thereby regulating gene transcription through epigenetic chromatin modification. This protein plays a role in diverse biological processes, including cellular differentiation, stem cell maintenance, and sex chromosome-linked gene regulation.
Structurally, JARID1D contains conserved domains such as ARID (DNA-binding domain) and multiple PHD zinc fingers, which mediate chromatin interaction and recruitment of co-factors. Notably, JARID1D is encoded on the Y chromosome and is implicated in male-specific physiological and pathological pathways, including spermatogenesis and androgen signaling. Dysregulation of JARID1D has been associated with cancers (e.g., prostate cancer), male infertility, and developmental disorders.
Recombinant human JARID1D protein is produced in vitro using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. Its recombinant form enables biochemical studies, such as enzymatic activity assays, interaction mapping with chromatin modifiers, and screening of epigenetic inhibitors. As a tool, it aids in dissecting sex-linked gene regulation and developing targeted therapies for Y chromosome-related diseases. The protein’s solubility, stability, and functional integrity make it valuable for structural and functional research in epigenetics.
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