| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JAKMIP1 |
| Uniprot No | Q96N16 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626aa |
| 活性数据 | MSKKGRSKGEKPEMETDAVQMANEELRAKLTSIQIEFQQEKSKVGKLRERLQEAKLEREQEQRRHTAYISELKAKLHEEKTKELQALREGLIRQHEQEAARTAKIKEGELQRLQATLNVLRDGAADKVKTALLTEAREEARRAFDGERLRLQQEILELKAARNQAEEALSNCMQADKTKAADLRAAYQAHQDEVHRIKRECERDIRRLMDEIKGKDRVILALEKELGVQAGQTQKLLLQKEALDEQLVQVKEAERHHSSPKRELPPGIGDMVELMGVQDQHMDERDVRRFQLKIAELNSVIRKLEDRNTLLADERNELLKRSRETEVQLKPLVEKNKRMNKKNEDLLQSIQRMEEKIKNLTRENVEMKEKLSAQASLKRHTSLNDLSLTRDEQEIEFLRLQVLEQQHVIDDLSLERERLLRSKRHRGKSLKPPKKHVVETFFGFDEESVDSETLSETSYNTDRTDRTPATPEEDLDDATAREEADLRFCQLTREYQALQRAYALLQEQVGGTLDAEREARTREQLQADLLRCQAKIEDLEKLLVEKGQDSKWVEEKQLLIRTNQDLLEKIYRLEMEENQLKNEMQDAKDQNELLEFRVLELEVRDSICCKLSNGADILFEPKLKFM |
| 分子量 | 68.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的关于重组人JAKMIP1蛋白的参考文献示例(请注意，实际文献可能需要通过学术数据库验证)：
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1. **文献名称**: "Cloning and Functional Characterization of Human JAKMIP1: A Novel Regulator of JAK-STAT Signaling"
**作者**: Smith A, et al.
**摘要**: 本研究利用哺乳动物表达系统成功克隆并纯化重组人JAKMIP1蛋白，证实其通过直接结合JAK激酶(如JAK3)负向调控JAK-STAT信号通路，并探究了其在免疫细胞中的抑制作用。
2. **文献名称**: "JAKMIP1 Modulates Microtubule Dynamics in Neuronal Development via Recombinant Protein Interaction Studies"
**作者**: Zhang L, et al.
**摘要**: 通过在大肠杆菌中表达重组JAKMIP1蛋白，研究者发现其通过与神经元微管蛋白结合调控细胞骨架动态，影响突触形成和神经元的极性迁移。
3. **文献名称**: "Structural Insights into JAKMIP1-JAK3 Interaction Domains Using Recombinant Protein Assays"
**作者**: Johnson R, et al.
**摘要**: 利用重组人JAKMIP1片段进行体外结合实验和X射线晶体学分析，揭示其C端结构域为JAK3结合的关键区域，为相关疾病(如免疫失调)的药物设计提供依据。
4. **文献名称**: "JAKMIP1 Knockdown Enhances Inflammatory Signaling: Evidence from Recombinant Overexpression Models"
**作者**: Lee H, et al.
**摘要**: 通过构建重组JAKMIP1过表达细胞模型，发现JAKMIP1通过抑制JAK激酶磷酸化降低IL-4/IL-13介导的炎症反应，提示其在过敏性疾病中的潜在治疗作用。
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**注意事项**：上述文献为示例性内容，实际研究中需通过 **PubMed**、**Google Scholar** 等平台以关键词“recombinant human JAKMIP1”“JAKMIP1 signaling”进行检索，并核对作者及发表年份的准确性。现有文献可能更侧重于JAKMIP1在神经发育或免疫调控中的机制探索。
Recombinant human JAKMIP1 (Janus kinase and microtubule-interacting protein 1) is a protein encoded by the *JAKMIP1* gene, primarily expressed in neural and immune tissues. It belongs to the JAKMIP family, characterized by conserved domains involved in protein-protein interactions and microtubule binding. JAKMIP1 regulates intracellular signaling pathways, particularly the JAK-STAT cascade, which mediates responses to cytokines and growth factors. It also interacts with microtubules, suggesting roles in cytoskeletal organization and intracellular transport. Dysregulation of JAKMIP1 has been linked to neurodevelopmental disorders, autoimmune conditions, and cancers, though its exact mechanisms remain under investigation.
The recombinant form is typically produced in *E. coli*, insect, or mammalian expression systems for research applications. Its purification enables studies on structure-function relationships, interaction partners, and therapeutic targeting. Researchers utilize recombinant JAKMIP1 to explore its regulatory effects on synaptic plasticity, immune cell activation, and oncogenic pathways. Ongoing work aims to clarify its dual roles in signaling modulation and cytoskeletal dynamics, with potential implications for treating neurological and inflammatory diseases. The protein's versatility in experimental models underscores its importance in deciphering complex cellular networks.
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