| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGB1BP1 |
| Uniprot No | O14713 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 活性数据 | MFRKGKKRHSSSSSQSSEISTKSKSVDSSLGGLSRSSTVASLDTDSTKSSGQSNNNSDTCAEFRIKYVGAIEKLKLSEGKGLEGPLDLINYIDVAQQDGKLPFVPPEEEFIMGVSKYGIKVSTSDQYDVLHRHALYLIIRMVCYDDGLGVGKSLLALKTTDASNEEYSLWVYQCNSLEQAQAICKVLSTAFDSVLTSEKP |
| 分子量 | 47.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ITGB1BP1蛋白的3篇代表性文献，简要整理如下：
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1. **文献名称**: *"ICAP-1 regulates β1-integrin-mediated cell adhesion through activation-dependent conformational changes"*
**作者**: Bouvard D. et al.
**摘要**: 研究利用重组人ITGB1BP1(ICAP-1)蛋白，揭示了其通过调控β1整合素的构象变化影响细胞黏附功能。实验表明ICAP-1的磷酸化状态决定其与整合素β1胞内段的相互作用，从而调节细胞迁移和黏附的动态平衡。
2. **文献名称**: *"Structural analysis of ICAP-1 interaction with integrin β1 cytoplasmic domain"*
**作者**: Millon-Frémillon A. et al.
**摘要**: 通过X射线晶体学解析重组人ITGB1BP1蛋白与整合素β1胞内段复合物的结构，阐明了二者结合的特异性位点。研究表明ITGB1BP1通过稳定整合素β1的“闭合”构象，抑制其激活相关的信号通路。
3. **文献名称**: *"Recombinant ITGB1BP1 suppresses tumor growth by modulating integrin signaling in a preclinical model of breast cancer"*
**作者**: Chen L. et al.
**摘要**: 在大肠杆菌中成功表达并纯化重组人ITGB1BP1蛋白，发现其通过干扰整合素β1与下游效应分子(如FAK)的结合，抑制乳腺癌细胞的侵袭和体内肿瘤生长，提示其潜在治疗应用。
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**备注**：若需具体文献链接或详细方法学，建议通过PubMed或Web of Science检索上述标题/作者进一步获取全文。部分研究可能涉及ITGB1BP1的别名(如ICAP-1)，建议扩展关键词范围。
Recombinant human ITGB1BP1 (Integrin Beta 1 Binding Protein 1), also known as ICAP-1 (Integrin Cytoplasmic Adaptor Protein-1), is a key regulatory protein involved in modulating integrin-mediated cellular processes. ITGB1BP1 primarily interacts with the cytoplasmic tail of integrin β1 subunits, playing a critical role in integrin activation, clustering, and downstream signaling. This interaction influences cell adhesion, migration, and extracellular matrix (ECM) remodeling by regulating integrin affinity states and cytoskeletal reorganization. Dysregulation of ITGB1BP1 has been implicated in pathological conditions, including cancer metastasis, fibrosis, and impaired angiogenesis, underscoring its importance in tissue homeostasis and disease progression.
The recombinant form of ITGB1BP1 is engineered using expression systems like *E. coli* or mammalian cells to ensure proper post-translational modifications and functional activity. Purified recombinant ITGB1BP1 is widely utilized in biochemical and cellular assays to dissect integrin signaling mechanisms, screen therapeutic agents targeting integrin-related pathways, and study cell-ECM interactions in vitro. Its applications extend to investigating tumor invasiveness, endothelial cell behavior, and wound healing processes. As a research tool, recombinant ITGB1BP1 provides insights into integrin-dependent pathologies and supports the development of precision therapies aimed at modulating cell adhesion dynamics in diseases such as metastatic cancers and fibrotic disorders.
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