| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ISYNA1 |
| Uniprot No | Q9NPH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 活性数据 | MEAAAQFFVESPDVVYGPEAIEAQYEYRTTRVSREGGVLKVHPTSTRFTFRTARQVPRLGVMLVGWGGNNGSTLTAAVLANRLRLSWPTRSGRKEANYYGSLTQAGTVSLGLDAEGQEVFVPFSAVLPMVAPNDLVFDGWDISSLNLAEAMRRAKVLDWGLQEQLWPHMEALRPRPSVYIPEFIAANQSARADNLIPGSRAQQLEQIRRDIRDFRSSAGLDKVIVLWTANTERFCEVIPGLNDTAENLLRTIELGLEVSPSTLFAVASILEGCAFLNGSPQNTLVPGALELAWQHRVFVGGDDFKSGQTKVKSVLVDFLIGSGLKTMSIVSYNHLGNNDGENLSAPLQFRSKEVSKSNVVDDMVQSNPVLYTPGEEPDHCVVIKYVPYVGDSKRALDEYTSELMLGGTNTLVLHNTCEDSLLAAPIMLDLALLTELCQRVSFCTDMDPEPQTFHPVLSLLSFLFKAPLVPPGSPVVNALFRQRSCIENILRACVGLPPQNHMLLEHKMERPGPSLKRVGPVAATYPMLNKKGPVPAATNGCTGDANGHLQEEPPMPTT |
| 分子量 | 87.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人ISYNA1(肌醇-3-磷酸合成酶1)蛋白的模拟参考文献，涵盖其功能研究和应用方向：
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1. **文献名称**：*"Cloning, Expression, and Characterization of Recombinant Human Inositol-3-Phosphate Synthase 1 (ISYNA1)"*
**作者**：Majumder, A. L. et al.
**摘要**：本研究成功将人ISYNA1基因克隆至大肠杆菌表达系统，纯化出带有His标签的重组蛋白。通过体外酶活实验，证实其催化葡萄糖-6-磷酸生成肌醇-3-磷酸的功能，并分析了温度与pH对酶活性的影响。
2. **文献名称**：*"Structural Insights into ISYNA1 Catalytic Mechanism by X-ray Crystallography"*
**作者**：Chakraborty, A. et al.
**摘要**：通过X射线晶体学解析了重组人ISYNA1蛋白的三维结构，揭示了其底物结合口袋及关键氨基酸残基(如Asp143和His267)在催化中的作用，为设计选择性抑制剂提供了结构基础。
3. **文献名称**：*"ISYNA1 Overexpression Alters Inositol Biosynthesis in Diabetic Cell Models"*
**作者**：Murakami, T. et al.
**摘要**：利用重组ISYNA1蛋白在HEK293细胞中过表达，发现其通过调节肌醇代谢通路影响胰岛素信号传导，提示ISYNA1在糖尿病病理中的潜在作用。
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**注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science搜索真实文献(关键词：ISYNA1. inositol synthase, recombinant expression)。
**Background of Recombinant Human ISYNA1 Protein**
The inositol-3-phosphate synthase 1 (ISYNA1), also known as myo-inositol-1-phosphate synthase (MIPS), is a key enzyme encoded by the *ISYNA1* gene in humans. It catalyzes the rate-limiting step in the de novo synthesis of myo-inositol, converting glucose-6-phosphate to inositol-3-phosphate, a precursor for phosphoinositides and inositol polyphosphates. These molecules play critical roles in cellular signaling, membrane trafficking, and osmoregulation. ISYNA1 is particularly vital in tissues with high inositol demand, such as the brain, kidney, and liver, where myo-inositol supports neurological function and metabolic homeostasis.
Recombinant human ISYNA1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional enzyme for research and therapeutic applications. Its study aids in understanding inositol biosynthesis defects linked to diseases like type 2 diabetes, bipolar disorder, and Alzheimer’s disease. Additionally, recombinant ISYNA1 enables screening of pharmacological modulators to target inositol-related pathways, offering potential interventions for metabolic and neurological disorders. Structural and functional analyses of the protein also provide insights into evolutionary conservation across species and mechanisms regulating cellular inositol levels under stress conditions.
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