| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRX6 |
| Uniprot No | P78412 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446aa |
| 活性数据 | MSFPHFGHPYRGASQFLASASSSTTCCESTQRSVSDVASGSTPAPALCCAPYDSRLLGSARPELGAALGIYGAPYAAAAAAQSYPGYLPYSPEPPSLYGALNPQYEFKEAAGSFTSSLAQPGAYYPYERTLGQYQYERYGAVELSGAGRRKNATRETTSTLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWAPKNKGGEERKAEGGEEDSLGCLTADTKEVTASQEARGLRLSDLEDLEEEEEEEEEAEDEEVVATAGDRLTEFRKGAQSLPGPCAAAREGRLERRECGLAAPRFSFNDPSGSEEADFLSAETGSPRLTMHYPCLEKPRIWSLAHTATASAVEGAPPARPRPRSPECRMIPGQPPASARRLSVPRDSACDESSCIPKAFGNPKFALQGLPLNCAPCPRRSEPVVQCQYPSGAEAG |
| 分子量 | 74.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IRX6蛋白的参考文献示例(注：以下内容为虚构，仅供参考)：
1. **文献名称**: "Recombinant Human IRX6 Protein Regulates Pancreatic Beta Cell Differentiation"
**作者**: Smith J, Brown A, Lee C
**摘要**: 本研究利用重组人IRX6蛋白探索其在胰腺发育中的功能，发现IRX6通过激活下游靶基因(如Pdx1和Nkx6.1)促进胰岛β细胞的分化，为糖尿病细胞治疗提供潜在靶点。
2. **文献名称**: "IRX6 Recombinant Protein Modulates Wnt Signaling in Colorectal Cancer"
**作者**: Wang L, Zhang Y, Kimura T
**摘要**: 通过体外实验证实，重组IRX6蛋白能够抑制结直肠癌细胞中Wnt/β-catenin信号通路的异常活化，从而降低肿瘤侵袭性，提示其在癌症治疗中的潜在应用。
3. **文献名称**: "Structural and Functional Characterization of Recombinant Human IRX6"
**作者**: Gupta S, Müller R, Schmidt H
**摘要**: 本研究解析了重组IRX6蛋白的晶体结构，揭示了其DNA结合域的关键氨基酸残基，并通过功能实验验证其调控靶基因的分子机制。
4. **文献名称**: "IRX6 Recombinant Protein Enhances Cardiomyocyte Regeneration in Zebrafish"
**作者**: Chen X, Tanaka M, Zhou D
**摘要**: 在斑马鱼模型中，重组IRX6蛋白通过激活心脏发育相关基因(如Gata4和Tbx5)，显著促进心肌细胞再生，为心脏损伤修复提供新思路。
(注：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等学术平台查询。)
Recombinant human IRX6 protein is derived from the Iroquois homeobox gene family member IRX6. which encodes a transcription factor involved in developmental processes. The Iroquois (IRX) genes are evolutionarily conserved and play critical roles in embryonic patterning, organogenesis, and tissue differentiation. IRX6 specifically is implicated in skeletal development, particularly in chondrogenesis and endochondral ossification, where it regulates the proliferation and differentiation of chondrocytes. Studies suggest its involvement in limb formation, craniofacial development, and vertebral patterning, with mutations or dysregulation linked to congenital disorders such as cleft palate and spinal defects.
IRX6 also contributes to pancreatic β-cell maturation and insulin secretion, highlighting its role in metabolic homeostasis. Its expression is tightly regulated during embryogenesis, often in coordination with other IRX family members. Recombinant IRX6 protein, produced via heterologous expression systems like E. coli or mammalian cells, enables functional studies in vitro. Researchers utilize it to investigate DNA-binding properties, protein-protein interactions, and downstream signaling pathways. Applications extend to disease modeling, drug discovery for skeletal or metabolic disorders, and regenerative medicine. Despite progress, the full spectrum of IRX6's biological functions and regulatory networks remains an active area of research, particularly in understanding its tissue-specific roles and therapeutic potential.
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