| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INTS7 |
| Uniprot No | Q9NVH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-962aa |
| 活性数据 | MASNSTKSFLADAGYGEQELDANSALMELDKGLRSGKLGEQCEAVVRFPRLFQKYPFPILINSAFLKLADVFRVGNNFLRLCVLKVTQQSEKHLEKILNVDEFVKRIFSVIHSNDPVARAITLRMLGSLASIIPERKNAHHSIRQSLDSHDNVEVEAAVFAAANFSAQSKDFAVGICNKISEMIQGLATPVDLKLKLIPILQHMHHDAILASSARQLLQQLVTSYPSTKMVIVSLHTFTLLAASSLVDTPKQIQLLLQYLKNDPRKAVKRLAIQDLKLLANKTPHTWSRENIQALCECALQTPYDSLKLGMLSVLSTLSGTIAIKHYFSIVPGNVSSSPRSSDLVKLAQECCYHNNRGIAAHGVRVLTNITVSCQEKDLLALEQDAVFGLESLLVLCSQDDSPGAQATLKIALNCMVKLAKGRPHLSQSVVETLLTQLHSAQDAARILMCHCLAAIAMQLPVLGDGMLGDLMELYKVIGRSATDKQQELLVSLATVIFVASQKALSVESKAVIKQQLESVSNGWTVYRIARQASRMGNHDMAKELYQSLLTQVASEHFYFWLNSLKEFSHAEQCLTGLQEENYSSALSCIAESLKFYHKGIASLTAASTPLNPLSFQCEFVKLRIDLLQAFSQLICTCNSLKTSPPPAIATTIAMTLGNDLQRCGRISNQMKQSMEEFRSLASRYGDLYQASFDADSATLRNVELQQQSCLLISHAIEALILDPESASFQEYGSTGTAHADSEYERRMMSVYNHVLEEVESLNRKYTPVSYMHTACLCNAIIALLKVPLSFQRYFFQKLQSTSIKLALSPSPRNPAEPIAVQNNQQLALKVEGVVQHGSKPGLFRKIQSVCLNVSSTLQSKSGQDYKIPIDNMTNEMEQRVEPHNDYFSTQFLLNFAILGTHNITVESSVKDANGIVWKTGPRTTIFVKSLEDPYSQQIRLQQQQAQQPLQQQQQRNAYTRF |
| 分子量 | 133.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人INTS7蛋白的参考文献示例(均为虚构示例，仅供参考)：
1. **文献名称**：*Role of Recombinant Human INTS7 in Transcriptional Regulation and DNA Repair*
**作者**：Smith J. et al.
**摘要**：本研究利用重组人INTS7蛋白揭示了其在RNA聚合酶II介导的转录调控中的作用，发现INTS7通过整合复合体参与DNA损伤修复通路，调控靶基因的转录暂停与重启。
2. **文献名称**：*INTS7 Knockdown Promotes Tumor Metastasis via Dysregulation of Alternative Splicing in Breast Cancer*
**作者**：Zhang L. et al.
**摘要**：通过重组INTS7蛋白体外实验，发现INTS7缺失导致乳腺癌细胞中异常剪接事件增加，激活促转移信号通路，提示INTS7可能作为癌症治疗的潜在靶点。
3. **文献名称**：*Structural Analysis of Recombinant INTS7 and Its Interaction with Integrator Complex Subunits*
**作者**：Brown K. et al.
**摘要**：该研究通过重组表达纯化人INTS7蛋白，结合冷冻电镜技术解析其三维结构，阐明INTS7与其他整合复合体亚基(如INTS3/5)的关键结合域，为功能机制提供结构基础。
4. **文献名称**：*Development of a High-throughput Screening Assay Using Recombinant INTS7 for Drug Discovery*
**作者**：Wang Y. et al.
**摘要**：构建了重组人INTS7蛋白的高效表达体系，并基于其酶活性建立了药物筛选平台，用于发现调控整合复合体功能的先导化合物。
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注：以上文献为模拟内容，实际研究中请通过PubMed、Web of Science等数据库检索真实发表的论文。
The recombinant human INTS7 protein is derived from the Integrator Complex Subunit 7. a critical component of the Integrator complex—a multi-subunit assembly involved in RNA polymerase II (RNAPII)-mediated transcription and RNA processing. The Integrator complex plays diverse roles in regulating gene expression, including the 3ʹ-end processing of small nuclear RNAs (snRNAs), enhancer RNA termination, and transcriptional elongation/pausing. INTS7. encoded by the *INTS7* gene on chromosome 1p36.11. is an evolutionarily conserved subunit implicated in stabilizing the complex and facilitating interactions with other transcriptional regulators. Dysregulation of INTS7 has been linked to developmental defects and diseases, such as cancer, underscoring its biological relevance.
Recombinant INTS7 protein is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its recombinant form allows researchers to probe molecular mechanisms underlying RNA processing, chromatin remodeling, and transcriptional regulation. Studies using recombinant INTS7 have shed light on its role in DNA repair pathways and cellular stress responses. Additionally, it serves as a tool for identifying binding partners or screening therapeutic agents targeting Integrator complex-related pathologies. Its application spans basic research, drug discovery, and biotechnological assays aimed at elucidating gene regulatory networks.
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