| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INTS5 |
| Uniprot No | Q6P9B9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1019aa |
| 活性数据 | MSALCDPPGAPGPPGPAPATHGPAPLSAQELSQEIKAFLTGVDPILGHQLSAREHARCGLLLLRSLPPARAAVLDHLRGVFDESVRAHLAALDETPVAGPPHLRPPPPSHVPAGGPGLEDVVQEVQQVLSEFIRANPKAWAPVISAWSIDLMGQLSSTYSGQHQRVPHATGALNELLQLWMGCRATRTLMDIYVQCLSALIGSCPDACVDALLDTSVQHSPHFDWVVAHIGSSFPGTIISRVLSCGLKDFCVHGGAGGGAGSSGGSSSQTPSTDPFPGSPAIPAEKRVPKIASVVGILGHLASRHGDSIRRELLRMFHDSLAGGSGGRSGDPSLQATVPFLLQLAVMSPALLGTVSGELVDCLKPPAVLSQLQQHLQGFPREELDNMLNLAVHLVSQASGAGAYRLLQFLVDTAMPASVITTQGLAVPDTVREACDRLIQLLLLHLQKLVHHRGGSPGEGVLGPPPPPRLVPFLDALKNHVGELCGETLRLERKRFLWQHQLLGLLSVYTRPSCGPEALGHLLSRARSPEELSLATQLYAGLVVSLSGLLPLAFRSCLARVHAGTLQPPFTARFLRNLALLVGWEQQGGEGPAALGAHFGESASAHLSDLAPLLLHPEEEVAEAAASLLAICPFPSEALSPSQLLGLVRAGVHRFFASLRLHGPPGVASACQLLTRLSQTSPAGLKAVLQLLVEGALHRGNTELFGGQVDGDNETLSVVSASLASASLLDTNRRHTAAVPGPGGIWSVFHAGVIGRGLKPPKFVQSRNQQEVIYNTQSLLSLLVHCCSAPGGTECGECWGAPILSPEAAKAVAVTLVESVCPDAAGAELAWPPEEHARATVERDLRIGRRFREQPLLFELLKLVAAAPPALCYCSVLLRGLLAALLGHWEASRHPDTTHSPWHLEASCTLVAVMAEGSLLPPALGNMHEVFSQLAPFEVRLLLLSVWGFLREHGPLPQKFIFQSERGRFIRDFSREGGGEGGPHLAVLHSVLHRNIDRLGLFSGRFQAPSPSTLLRQGT |
| 分子量 | 134.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人INTS5蛋白的3篇参考文献(示例基于假设性研究文献，供参考)：
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1. **文献名称**：*INTS5 Regulates mRNA Processing and Cell Cycle Progression*
**作者**：Zhang Y et al.
**摘要**：研究揭示了INTS5作为整合复合体(Integrator complex)亚基的功能，通过重组人INTS5蛋白实验证实其参与RNA聚合酶II依赖性转录调控，并影响细胞周期G1/S期的转换。
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2. **文献名称**：*CRISPR Screening Identifies INTS5 as a Mediator of DNA Damage Response*
**作者**：Lee S, Kim J.
**摘要**：利用重组人INTS5蛋白及基因编辑技术，发现INTS5在DNA损伤修复中发挥重要作用，其缺失导致基因组不稳定性和转录延伸异常。
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3. **文献名称**：*Structural and Functional Analysis of Human INTS5 in snRNA 3’-End Processing*
**作者**：Mendoza-Figueroa MS et al.
**摘要**：通过重组表达并纯化人INTS5蛋白，结合结构生物学分析，阐明了INTS5在snRNA 3’端加工中的分子机制及其与其他整合复合体亚基的相互作用。
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**备注**：以上文献为模拟示例，实际文献需通过PubMed、Google Scholar等数据库检索。若需具体文献，建议以关键词“INTS5 recombinant human protein”或“Integrator complex subunit 5”查询近年研究。
The integrator complex subunit 5 (INTS5) is a key component of the Integrator complex, a multi-protein assembly associated with RNA polymerase II (RNAP II). This complex plays a critical role in transcription regulation, 3' end processing of small nuclear RNAs (snRNAs), and select mRNA biogenesis. INTS5. also known as DRE4 or C5orf30. is evolutionarily conserved and interacts with other Integrator subunits to mediate RNA processing and transcriptional termination.
Recombinant human INTS5 protein is engineered for in vitro studies to dissect its molecular functions. Produced via expression systems like *E. coli* or mammalian cells, it retains functional domains for binding RNA or partner proteins. Research using recombinant INTS5 has highlighted its involvement in snRNA maturation and transcriptional pausing, linking it to cellular processes such as splicing, cell cycle progression, and stress responses.
Dysregulation of INTS5 has been implicated in diseases, including cancers and neurodevelopmental disorders. Recombinant INTS5 serves as a tool to study mutations, protein interactions, and mechanisms underlying these pathologies. Its structural and functional characterization aids in understanding Integrator-mediated gene regulation, offering insights into therapeutic targeting of RNAP II-dependent transcriptional anomalies.
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