| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INTS3 |
| Uniprot No | Q68E01 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1042aa |
| 活性数据 | MELQKGKGAAAAAAASGAAGGGGGGAGAGAPGGGRLLLSTSLDAKDELEERLERCMSIVTSMTAGVSEREANDALNAYVCKGLPQHEEICLGLFTLILTEPAQAQKCYRDLALVSRDGMNIVLNKINQILMEKYLKLQDTCRTQLVWLVRELVKSGVLGADGVCMTFMKQIAGGDVTAKNIWLAESVLDILTEQREWVLKSSILIAMAVYTYLRLIVDHHGTAQLQALRQKEVDFCISLLRERFMECLMIGRDLVRLLQNVARIPEFELLWKDIIHNPQALSPQFTGILQLLQSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTPDSQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFSPDKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPNFYPPLEGHVRQGVFSSLNHIVEKRVLAHLAPLFDNPKLDKELRAMLREKFPEFCSSPSPPVEVKIEEPVSMEMDNHMSDKDESCYDNAEAAFSDDEEDLNSKGKKREFRFHPIKETVVEEPVDITPYLDQLDESLRDKVLQLQKGSDTEAQCEVMQEIVDQVLEEDFDSEQLSVLASCLQELFKAHFRGEVLPEEITEESLEESVGKPLYLIFRNLCQMQEDNSSFSLLLDLLSELYQKQPKIGYHLLYYLRASKAAAGKMNLYESFAQATQLGDLHTCLMMDMKACQEDDVRLLCHLTPSIYTEFPDETLRSGELLNMIVAVIDSAQLQELVCHVMMGNLVMFRKDSVLNILIQSLDWETFEQYCAWQLFLAHNIPLETIIPILQHLKYKEHPEALSCLLLQLRREKPSEEMVKMVLSRPCHPDDQFTTSILRHWCMKHDELLAEHIKSLLIKNNSLPRKRQSLRSSSSKLAQLTLEQILEHLDNLRLNLTNTKQNFFSQTPILQALQHVQASCDEAHKMKFSDLFSLAEEYEDSSTKPPKSRRKAALSSPRSRKNATQPPNAEEESGSSSASEEEDTKPKPTKRKRKGSSAVGSDSD |
| 分子量 | 144.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人INTS3蛋白的模拟参考文献示例(请注意，这些文献为虚构示例，仅供参考)：
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1. **文献名称**: "Recombinant Human INTS3 Facilitates DNA Repair via Homologous Recombination"
**作者**: Smith A, et al.
**摘要**: 研究通过在大肠杆菌中重组表达人INTS3蛋白，揭示了其在DNA损伤应答中的作用。实验表明INTS3与RAD51相互作用，促进同源重组修复，缺失INTS3导致细胞对电离辐射敏感。
2. **文献名称**: "Structural Characterization of the INTS3 Protein in the Integrator Complex"
**作者**: Johnson B, et al.
**摘要**: 利用昆虫细胞系统表达重组INTS3蛋白，结合冷冻电镜解析了INTS3在整合复合体中的三维结构，阐明了其参与RNA聚合酶II依赖性小核RNA加工的分子机制。
3. **文献名称**: "INTS3 Regulates Cell Cycle Progression through Interaction with C9ORF80"
**作者**: Gomez D, et al.
**摘要**: 通过共表达重组INTS3与C9ORF80蛋白，发现二者形成稳定复合物，调控G1/S期转换。敲低INTS3导致细胞周期阻滞，提示其在增殖性疾病中的潜在作用。
4. **文献名称**: "Functional Redundancy of INTS3 in snRNA 3'-End Processing"
**作者**: Lee C, et al.
**摘要**: 研究利用体外重组系统证明INTS3与整合复合体其他亚基协同作用，确保小核RNA(snRNA)的正确剪切和多聚腺苷酸化，缺失时引发转录组异常。
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**注意**：以上文献及内容均为模拟生成，实际研究中请通过学术数据库检索真实文献。如需具体文献推荐，建议使用关键词“recombinant INTS3”或“INTS3 function”在PubMed、Google Scholar等平台查询。
The recombination human INTS3 protein (recombinant human INTS3) is a genetically engineered form of the integrator complex subunit 3. a critical component of the Integrator complex. This multisubunit complex plays diverse roles in RNA processing, transcriptional regulation, and DNA damage response. INTS3 specifically associates with other Integrator subunits (INTS1. INTS4. INTS5. etc.) to mediate 3′ end processing of small nuclear RNAs (snRNAs) and enhancer RNAs, while also participating in resolving R-loop-associated replication stress to maintain genomic stability.
Studies link INTS3 dysfunction to developmental defects and cancer progression. Its interaction with tumor suppressors like BRCA1 and involvement in homologous recombination repair highlight its significance in DNA damage response pathways. Recombinant human INTS3 is typically expressed in bacterial or mammalian systems, often fused with affinity tags (e.g., His, GST) for purification. It serves as a vital tool for structural studies, biochemical assays (e.g., protein interaction mapping), and functional analyses in cellular models.
Current research focuses on elucidating its dual roles in RNA metabolism and genome integrity, with implications for understanding carcinogenesis and developing targeted therapies. Its recombinant form enables mechanistic insights into Integrator complex dynamics and disease-associated mutations.
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