| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INTS12 |
| Uniprot No | Q96CB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 活性数据 | MAATVNLELD PIFLKALGFL HSKSKDSAEK LKALLDESLA RGIDSSYRPS QKDVEPPKIS STKNISIKQE PKISSSLPSG NNNGKVLTTE KVKKEAEKRP ADKMKSDITE GVDIPKKPRL EKPETQSSPI TVQSSKDLPM ADLSSFEETS ADDFAMEMGL ACVVCRQMMV ASGNQLVECQ ECHNLYHRDC HKPQVTDKEA NDPRLVWYCA RCTRQMKRMA QKTQKPPQKP APAVVSVTPA VKDPLVKKPE TKLKQETTFL AFKRTEVKTS TVISGNSSSA SVSSSVTSGL TGWAAFAAKT SSAGPSTAKL SSTTQNNTGK PATSSANQKP VGLTGLATSS KGGIGSKIGS NNSTTPTVPL KPPPPLTLGK TGLSRSVSCD NVSKVGLPSP SSLVPGSSSQ LSGNGNSGTS GPSGSTTSKT TSESSSSPSA SLKGPTSQES QLNAMKRLQM VKKKAAQKKL KK |
| 分子量 | 48.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人INTS12蛋白的参考文献及其核心内容概括：
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1. **文献名称**: *The Integrator complex subunit 12 is required for RNA splicing and neural development in humans*
**作者**: Albrecht TR, et al.
**摘要**: 本研究揭示了INTS12在Integrator复合体中的关键作用，通过重组人INTS12蛋白的表达和功能验证，证明其与RNA剪接和胚胎神经发育相关，突变会导致剪接缺陷和神经系统疾病。
2. **文献名称**: *Structural and functional analysis of the human Integrator-INTS12 interface*
**作者**: Chen FX, et al.
**摘要**: 通过重组人INTS12蛋白的体外重构实验，解析了INTS12与Integrator复合体其他亚基(如INTS4和INTS5)的互作机制，并发现其磷酸化修饰对RNA聚合酶II的转录终止调控至关重要。
3. **文献名称**: *INTS12 promotes colorectal cancer progression by enhancing c-Myc mRNA stability*
**作者**: Li Y, et al.
**摘要**: 研究利用重组人INTS12蛋白进行蛋白质-RNA结合实验，发现INTS12通过结合c-Myc mRNA的3’UTR区域调控其稳定性，进而促进结直肠癌细胞增殖和转移。
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**注**：以上文献为示例性内容，实际文献可能需通过PubMed或Web of Science检索关键词“recombinant human INTS12”或“INTS12 function”获取。如需具体文献DOI，请提供更详细的研究方向。
The human INTS12 protein is a component of the Integrator complex, a multi-subunit assembly involved in RNA polymerase II (RNAPII)-mediated transcriptional regulation. This complex plays critical roles in RNA processing, including the 3' end maturation of small nuclear RNAs (snRNAs) and enhancer RNAs (eRNAs), as well as modulating transcriptional pausing and termination. INTS12 specifically associates with other Integrator subunits (e.g., INTS3. INTS6) to facilitate its recruitment to chromatin, where it participates in DNA repair processes and transcriptional elongation control. Dysregulation of INTS12 has been linked to developmental disorders and cancer progression, underscoring its biological significance.
Recombinant human INTS12 protein is typically produced using heterologous expression systems (e.g., Escherichia coli or mammalian cells) for functional studies. Its purified form enables in vitro investigations into protein-protein interactions, structural analysis, and enzymatic activities within the Integrator complex. Researchers employ it to dissect mechanisms underlying transcriptional termination, RNA processing fidelity, and DNA damage response pathways. Additionally, recombinant INTS12 serves as a tool for screening therapeutic compounds targeting Integrator-mediated processes in diseases like melanoma or glioblastoma. Structural characterization of this protein (e.g., via crystallography or cryo-EM) further aids in mapping functional domains critical for its role in chromatin-associated processes.
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