| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INPP5B |
| Uniprot No | P32019 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-748aa |
| 活性数据 | MDQSVAIQETLAEGEYCVIAVQGVLCEGDSRQSRLLGLVRYRLEHGGQEHALFLYTHRRMAITGDDVSLDQIVPVSRDFTLEEVSPDGELYILGSDVTVQLDTAELSLVFQLPFGSQTRMFLHEVARACPGFDSATRDPEFLWLSRYRCAELELEMPTPRGCNSALVTWPGYATIGGGGSNFDGLRPNGKGVPMDQSSRGQDKPESLQPRQNKSKSEITDMVRSSTITVSDKAHILSMQKFGLRDTIVKSHLLQKEEDYTYIQNFRFFAGTYNVNGQSPKECLRLWLSNGIQAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSEGLHPDAKYAKVKLIRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQFHNTSICVVNSHLAAHIEEYERRNQDYKDICSRMQFCQPDPSLPPLTISNHDVILWLGDLNYRIEELDVEKVKKLIEEKDFQMLYAYDQLKIQVAAKTVFEGFTEGELTFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNDELYRKTLEEIVRSLDKMENANIPSVSLSKREFCFQNVKYMQLKVESFTIHNGQVPCHFEFINKPDEESYCKQWLNANPSRGFLLPDSDVEIDLELFVNKTTATKLNSGEDKIEDILVLHLDRGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLETISELLAYLAAYCFETQLVTKSLI |
| 分子量 | 111.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人INPP5B蛋白的3-4条参考文献示例(注：以下为虚拟文献，实际引用时请核实真实来源):
1. **"Expression and functional characterization of recombinant human INPP5B phosphatase"**
*Smith A, et al. (2003)*
研究利用杆状病毒-昆虫细胞系统表达重组人INPP5B蛋白，并验证其特异性水解磷脂酰肌醇3.4.5-三磷酸(PI(3.4.5)P3)的活性，揭示其在内体运输和高尔基体功能中的作用。
2. **"Structural insights into the catalytic mechanism of human INPP5B"**
*Jones C, et al. (2007)*
通过X射线晶体学解析重组INPP5B的晶体结构，鉴定其磷酸酶结构域的活性位点关键残基，并证实其底物结合特异性与调控细胞内肌醇信号通路的关系。
3. **"INPP5B regulates endosomal trafficking via dephosphorylation of PtdIns(3)P in HeLa cells"**
*Lee H, et al. (2011)*
该研究在大肠杆菌中重组表达并纯化INPP5B，结合细胞实验证明其通过水解PtdIns(3)P调控内体分选复合体(ESCRT)介导的膜运输过程，影响细胞器动态平衡。
4. **"Loss of INPP5B promotes cancer metastasis through dysregulated EGFR signaling"**
*Brown K, et al. (2015)*
利用重组INPP5B蛋白进行体外磷酸酶活性分析，发现其通过负向调控EGFR/Akt信号通路抑制肿瘤细胞迁移，为癌症靶向治疗提供新思路。
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**注**：上述文献为示例性质，若需引用真实论文，建议通过PubMed或Web of Science以“INPP5B”、“recombinant protein”、“inositol phosphatase”等关键词检索近年研究。
**Background of Recombinant Human INPP5B Protein**
Recombinant human INPP5B (Inositol Polyphosphate-5-Phosphatase B) is a purified protein derived from engineered expression systems, designed to study the enzymatic and regulatory roles of INPP5B in cellular processes. INPP5B belongs to the 5-phosphatase family, which hydrolyzes the 5-position phosphate from inositol phospholipids (e.g., PIP3) and inositol phosphates (e.g., IP3), modulating critical signaling pathways. It shares structural homology with the OCRL protein, mutations in which cause Lowe syndrome, but differs in subcellular localization and substrate specificity.
INPP5B is implicated in regulating membrane trafficking, cytoskeletal dynamics, and organelle function, particularly in the Golgi apparatus and endosomal compartments. It interacts with Rab GTPases, linking phosphoinositide metabolism to vesicle transport. Dysregulation of INPP5B has been associated with cancers, neurodevelopmental disorders, and cilia-related diseases, though its exact mechanisms remain under investigation.
The recombinant form, often produced in mammalian or insect cell systems, enables biochemical studies, structural analysis, and disease modeling. Its production allows controlled exploration of substrate interactions, enzyme kinetics, and therapeutic targeting. Researchers utilize it to dissect pathways involving phosphoinositide signaling, offering insights into cellular homeostasis and potential interventions for related pathologies.
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