| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INO80E |
| Uniprot No | Q8NBZ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-244aa |
| 氨基酸序列 | MNGPADGEVDYKKKYRNLKRKLKFLIYEHECFQEELRKAQRKLLKVSRDKSFLLDRLLQYENVDEDSSDSDATASSDNSETEGTPKLSDTPAPKRKRSPPLGGAPSPSSLSLPPSTGFPLQASGVPSPYLSSLASSRYPPFPSDYLALQLPEPSPLRPKREKRPRLPRKLKMAVGPPDCPVGGPLTFPGRGSGAGVGTTLTPLPPPKMPPPTILSTVPRQMFSDAGSGDDALDGDDDLVIDIPE |
| 分子量 | 52.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人INO80E蛋白的假设性参考文献示例，基于该蛋白可能的研究方向及已知复合体功能整理而成。若需实际文献，建议通过学术数据库进一步检索确认：
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1. **文献名称**：*Structural and functional characterization of the INO80 chromatin remodeling complex*
**作者**：Aygun, O., et al.
**摘要**：本研究解析了人源INO80复合体的结构，并通过重组表达技术纯化了包括INO80E在内的多个亚基。实验表明，INO80E在复合体组装和染色质重塑活性中起关键作用，重组蛋白的体外功能分析揭示了其对DNA修复通路的影响。
2. **文献名称**：*Recombinant expression and interaction mapping of INO80 subunits*
**作者**：Jin, J., et al.
**摘要**：作者通过大肠杆菌和昆虫细胞系统重组表达了人INO80复合体各亚基(含INO80E)，并利用pull-down实验验证亚基间的相互作用。研究发现INO80E作为支架蛋白，促进复合体稳定性，并通过体外ATP酶活性实验证明其对染色质重塑功能的贡献。
3. **文献名称**：*INO80E regulates transcriptional coactivation via histone variant exchange*
**作者**：Sasaki, K., & Peterson, C.L.
**摘要**：该研究利用重组人INO80E蛋白进行体外染色质重构实验，发现其通过介导组蛋白变体H2A.Z的交换参与基因转录调控。重组蛋白的突变分析揭示了INO80E特定结构域对功能的重要性。
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**备注**：上述文献为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“recombinant human INO80E”、“INO80 complex expression”)获取。建议参考近年关于染色质重塑复合体或DNA修复机制的研究，重点关注方法学中涉及重组蛋白表达的部分。
Recombinant human INO80E protein is a engineered form of the INO80E subunit, a critical component of the INO80 chromatin remodeling complex. The INO80 complex belongs to the SWI/SNF family of ATP-dependent chromatin remodelers, which regulate DNA accessibility by altering nucleosome positioning. INO80E (also known as Ies6 in yeast) specifically plays a role in stabilizing the complex’s structure and modulating its enzymatic activity during DNA repair, replication, and transcriptional regulation. Studies suggest its involvement in maintaining genomic stability through homology-directed repair of DNA double-strand breaks and replication fork restart.
The recombinant version is typically expressed in mammalian systems (e.g., HEK293 cells) with affinity tags for purification, enabling biochemical and structural studies. Its production allows researchers to investigate INO80 complex assembly, ATPase activity, and nucleosome-binding properties in vitro. Interest in INO80E has grown due to emerging links between chromatin remodeling defects and diseases, including cancers and developmental disorders. Researchers use this protein to explore how INO80-mediated chromatin dynamics influence epigenetic regulation, replication stress responses, and oncogenesis. Recent work also examines its potential as a therapeutic target, particularly in malignancies with compromised DNA repair pathways. The recombinant tool facilitates drug screening and mechanistic studies of chromatin remodeling in human disease models.
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