| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ING5 |
| Uniprot No | Q8WYH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-240aa |
| 氨基酸序列 | MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGKWFCPRCVQEKRKKK |
| 分子量 | 54.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人ING5蛋白相关的文献及其摘要概括(注：部分为示例性文献，需根据实际研究补充完整信息)：
1. **《ING5 suppresses cell proliferation and induces apoptosis in human breast cancer》**
- **作者**: Li X. et al. (2018)
- **摘要**: 研究重组人ING5蛋白在乳腺癌细胞中的过表达效应，发现其通过调控p53通路抑制细胞增殖并诱导细胞周期停滞和凋亡，提示ING5作为潜在肿瘤抑制因子的作用。
2. **《Recombinant human ING5 protein enhances histone acetylation and DNA damage response》**
- **作者**: Zhang Y. et al. (2020)
- **摘要**: 报道通过大肠杆菌表达系统成功纯化重组人ING5蛋白，证明其通过与组蛋白H3/H4结合，增强组蛋白乙酰转移酶活性，促进DNA损伤修复的分子机制。
3. **《Structural insights into the tumor-suppressive function of ING5 in epigenetic regulation》**
- **作者**: Wang L. et al. (2021)
- **摘要**: 利用X射线晶体学解析重组人ING5蛋白的结构，揭示其PHD锌指结构域与组蛋白H3K4me3的结合模式，为ING5通过表观遗传修饰调控基因表达的机制提供结构基础。
4. **《ING5 synergizes with chemotherapeutic agents by modulating p53 signaling in colorectal cancer》**
- **作者**: Chen R. et al. (2019)
- **摘要**: 研究重组ING5蛋白与化疗药物联用的效果，发现其通过增强p53的转录活性抑制结直肠癌细胞存活，并提高对5-FU的敏感性，提示联合治疗潜力。
(注：以上文献名称及内容为模拟示例，实际引用需核实具体研究并补充DOI/期刊信息。)
**Background of Recombinant Human ING5 Protein**
The INhibitor of Growth (ING) family, including ING5. comprises tumor suppressors involved in chromatin remodeling, cell cycle regulation, and apoptosis. ING5. a nuclear protein, acts as a critical component of histone acetyltransferase (HAT) complexes, such as MOZ/MORF, facilitating site-specific histone acetylation to regulate gene expression epigenetically. It interacts with p53 and other stress-responsive pathways, modulating DNA repair, cellular senescence, and genomic stability. Dysregulation of ING5 is linked to cancers, including breast, gastric, and hepatocellular carcinomas, where its downregulation often correlates with poor prognosis.
Recombinant human ING5 protein is engineered via prokaryotic or eukaryotic expression systems, enabling studies on its structural and functional roles. This purified protein retains binding affinity for histones and HAT complexes, making it valuable for *in vitro* assays, protein interaction studies, and investigating its tumor-suppressive mechanisms. Researchers utilize it to explore therapeutic strategies targeting epigenetic dysregulation in cancer or aging-related diseases. Additionally, recombinant ING5 aids in developing diagnostic tools by assessing its expression patterns or post-translational modifications in clinical samples. Its study contributes to understanding the interplay between chromatin dynamics, cellular stress responses, and carcinogenesis.
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