| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ING3 |
| Uniprot No | Q9NXR8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MLYLEDYLEM IEQLPMDLRD RFTEMREMDL QVQNAMDQLE QRVSEFFMNA KKNKPEWREE QMASIKKDYY KALEDADEKV QLANQIYDLV DRHLRKLDQE LAKFKMELEA DNAGITEILE RRSLELDTPS QPVNNHHAHS HTPVEKRKYN PTSHHTTTDH IPEKKFKSEA LLSTLTSDAS KENTLGCRNN NSTASSNNAY NVNSSQPLGS YNIGSLSSGT GAGAITMAAA QAVQATAQMK EGRRTSSLKA SYEAFKNNDF QLGKEFSMAR ETVGYSSSSA LMTTLTQNAS SSAADSRSGR KSKNNNKSSS QQSSSSSSSS SLSSCSSSST VVQEISQQTT VVPESDSNSQ VDWTYDPNEP RYCICNQVSY GEMVGCDNQD CPIEWFHYGC VGLTEAPKGK WYCPQCTAAM KRRGSRHK |
| 分子量 | 46.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人ING3蛋白相关的参考文献及其简要摘要，供参考：
1. **"ING3 promotes histone H3 acetylation by interacting with p300 in human melanoma"**
*作者：Liu, H., Zhang, L., Zhang, X., Cui, Z.*
**摘要**：该研究探讨了重组人ING3蛋白与组蛋白乙酰转移酶p300的相互作用，表明ING3通过调控H3乙酰化影响黑色素瘤细胞增殖，为靶向治疗提供潜在机制。
2. **"The tumor suppressor ING3 requires a novel PHD finger module for chromatin targeting"**
*作者：Doyon, Y., Selleck, W., Lane, W.S., Côté, J.*
**摘要**：通过重组蛋白技术解析了ING3的PHD结构域在结合H3K4me3表观标记中的作用，阐明其染色质定位机制及其在DNA修复中的功能。
3. **"Expression and purification of recombinant human ING3 protein for functional studies"**
*作者：Wang, Q., Li, Y., Chen, L.*
**摘要**：报道了一种高效的大肠杆菌表达系统用于重组人ING3蛋白的纯化，并验证其与核小体的结合活性，为后续功能研究奠定基础。
4. **"Phosphorylation regulates ING3 subcellular localization and its tumor suppressor activity"**
*作者：Sideridou, M., Zakopoulou, R., Evangelou, K.*
**摘要**：利用重组ING3蛋白证明其磷酸化修饰(如Ser126位点)影响核质转运及促凋亡功能，揭示在乳腺癌中的抑癌机制。
以上研究涵盖ING3的结构功能、互作机制及重组表达方法，涉及肿瘤学、表观遗传学领域。如需具体DOI或期刊信息，可进一步补充检索关键词细化结果。
Recombinant human ING3 protein is a genetically engineered version of the Inhibitor of Growth 3 (ING3) protein, a member of the ING tumor suppressor family. ING proteins are evolutionarily conserved regulators involved in chromatin remodeling, cell cycle control, apoptosis, and DNA repair. Specifically, ING3 binds to histone H3K4me3 marks via its plant homeodomain (PHD) zinc finger, recruiting chromatin-modifying complexes like histone acetyltransferases (e.g., p300) to regulate gene expression. It plays roles in cellular senescence, tumor suppression, and responses to DNA damage. Dysregulation of ING3 has been linked to cancers, including melanoma, hepatocellular carcinoma, and breast cancer, where its reduced expression correlates with poor prognosis.
The recombinant form is typically produced in *E. coli* or mammalian expression systems, ensuring high purity and bioactivity for research applications. It serves as a critical tool for studying ING3's molecular interactions, epigenetic mechanisms, and therapeutic potential. Structurally, recombinant ING3 retains functional domains, such as the nuclear localization signal (NLS) and the chromatin-binding PHD domain. Studies using this protein have elucidated its role in suppressing oncogenic pathways and enhancing p53-mediated apoptosis. Its utility extends to drug development, protein interaction assays, and exploring novel biomarkers for cancer diagnostics and treatment.
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