| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INCENP |
| Uniprot No | Q9NQS7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-918aa |
| 氨基酸序列 | MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAASSPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH |
| 分子量 | 105.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人INCENP蛋白的文献示例(模拟示例，具体文献请以实际数据库为准)：
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1. **文献名称**：Structure and Function of the Human INCENP Protein in Chromosomal Passenger Complex
**作者**：Adams RR et al.
**摘要**：研究通过重组表达人INCENP蛋白片段，结合X射线晶体学揭示了其与Aurora B激酶的相互作用结构域，阐明了其在调控染色体分离和胞质分裂中的分子机制。
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2. **文献名称**：Recombinant Production of Human INCENP for Kinase Activity Assays
**作者**：Sessa F et al.
**摘要**：报道一种高效的大肠杆菌表达系统，成功纯化重组人INCENP蛋白，并验证其与Aurora B激酶结合后显著增强后者的催化活性，为抗癌药物筛选奠定基础。
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3. **文献名称**：Dynamic Localization of Recombinant INCENP during Mitosis
**作者**：Bishop JD et al.
**摘要**：通过荧光标记的重组人INCENP蛋白活细胞成像，揭示其从染色体着丝粒向纺锤体中央区域的动态转位过程，证实其对末期胞质分裂的关键调控作用。
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**备注**：以上为模拟示例，实际文献需通过PubMed或Google Scholar以关键词“recombinant human INCENP”或“INCENP Aurora B interaction”检索。
Recombinant human INCENP (Inner Centromere Protein) is a key component of the chromosomal passenger complex (CPC), a critical regulator of mitotic and meiotic cell division. The CPC, which also includes Aurora B kinase, Survivin, and Borealin, orchestrates essential events such as chromosome alignment, kinetochore-microtubule attachment, cytokinesis, and the spindle assembly checkpoint. INCENP serves as a scaffolding protein that stabilizes the CPC and directly binds Aurora B, enhancing its kinase activity. Structurally, INCENP contains an N-terminal microtubule-binding domain, a central coiled-coil region mediating CPC assembly, and a C-terminal IN-box domain required for Aurora B activation.
Recombinant INCENP is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for in vitro studies to dissect CPC mechanisms. It enables researchers to explore interactions within the CPC, phosphorylation events regulating mitosis, and Aurora B’s role in correcting erroneous kinetochore attachments. Dysregulation of INCENP or the CPC is linked to chromosomal instability, aneuploidy, and cancer, making recombinant INCENP a valuable tool for probing cell cycle regulation and developing therapeutic strategies targeting mitotic errors. Its use in biochemical assays, structural studies, and drug screening underscores its importance in understanding genome integrity and disease pathology.
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