| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IMP4 |
| Uniprot No | Q96G21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291aa |
| 氨基酸序列 | MLRREARLRREYLYRKAREEAQRSAQERKERLRRALEENRLIPTELRREALALQGSLEFDDAGGEGVTSHVDDEYRWAGVEDPKVMITTSRDPSSRLKMFAKELKLVFPGAQRMNRGRHEVGALVRACKANGVTDLLVVHEHRGTPVGLIVSHLPFGPTAYFTLCNVVMRHDIPDLGTMSEAKPHLITHGFSSRLGKRVSDILRYLFPVPKDDSHRVITFANQDDYISFRHHVYKKTDHRNVELTEVGPRFELKLYMIRLGTLEQEATADVEWRWHPYTNTARKRVFLSTE |
| 分子量 | 60.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IMP4蛋白的3篇假设性参考文献示例(实际文献需根据真实数据库检索)：
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1. **文献名称**：**"The role of recombinant human IMP4 in ribosome biogenesis and rRNA processing"**
**作者**：Xia L, et al.
**摘要**：本文通过重组表达人源IMP4蛋白，揭示了其在核糖体RNA加工中的作用机制，证明IMP4通过与SSU-rRNA结合，调控前体rRNA的剪接和成熟过程，对核糖体亚基组装至关重要。
2. **文献名称**：**"Structural analysis of recombinant hIMP4 reveals RNA-binding motifs critical for its function"**
**作者**：Liu Y, Chen H.
**摘要**：利用X射线晶体学解析了重组人IMP4蛋白的三维结构，发现其含有保守的KH结构域，并通过体外实验验证了该结构域与RNA结合的能力，为阐明IMP4在核糖体合成中的分子机制提供依据。
3. **文献名称**：**"Overexpression of recombinant IMP4 promotes hepatocellular carcinoma progression via mTOR signaling activation"**
**作者**：Wang Q, et al.
**摘要**：研究通过重组IMP4蛋白在肝癌细胞中的过表达，证实其通过激活mTOR通路促进肿瘤细胞增殖和迁移，提示IMP4可能作为肝癌治疗的潜在靶点。
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(注：以上文献为示例，若需真实文献，请通过PubMed或Google Scholar以关键词“IMP4”、“hnRNP”、“ribosome biogenesis”等检索。)
Recombinant human IMP4 protein is a genetically engineered form of the IMP4 (Involved in Metabolism and Proliferation 4) protein, which plays a critical role in ribosome biogenesis and cellular proliferation. IMP4. also known as HTRA3. is a member of the Brix domain-containing protein family and is evolutionarily conserved across eukaryotes. It is primarily localized in the nucleolus, where it participates in the processing and maturation of 18S ribosomal RNA (rRNA) during the assembly of the 40S ribosomal subunit. Structurally, IMP4 contains KH and SAF (scaffold-associated factor) domains, which facilitate RNA binding and interactions with other ribosome assembly factors.
Aberrant IMP4 expression has been linked to various cancers, including hepatocellular carcinoma and gastric cancer, where its overexpression correlates with tumor progression and poor prognosis. Studies suggest that IMP4 supports cell cycle progression by regulating ribosome synthesis, which is essential for rapid cell division in cancerous tissues.
Recombinant IMP4 is typically produced using bacterial or mammalian expression systems, enabling researchers to study its molecular interactions, structural features, and regulatory mechanisms in vitro. This protein serves as a valuable tool for investigating ribosomopathies, cancer biology, and potential therapeutic targets. Its application extends to biochemical assays, antibody production, and functional studies exploring RNA-protein interactions and nucleolar dynamics.
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