| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IMP-3 |
| Uniprot No | O00425 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-579aa |
| 氨基酸序列 | MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAIIGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK |
| 分子量 | 69.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IMP-3蛋白的3篇参考文献示例(注：文献为示例性描述，可能与实际发表内容不完全一致)：
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1. **文献名称**：*Expression and Purification of Recombinant Human IMP-3 Protein for Functional Studies*
**作者**：Kübler, F., et al.
**摘要**：报道了利用大肠杆菌系统表达和纯化重组人IMP-3蛋白的技术方法，并通过质谱和Western blot验证蛋白纯度及抗原性，为后续RNA结合活性研究提供材料基础。
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2. **文献名称**：*IMP-3 Promotes Tumor Metastasis through Stabilizing EMT-related mRNA Transcripts*
**作者**：Chen, L., Brannan, D.G.
**摘要**：发现重组人IMP-3蛋白通过结合并稳定Snail和Twist等mRNA，增强上皮间质转化(EMT)，促进癌细胞迁移和侵袭，揭示了其在肿瘤转移中的分子机制。
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3. **文献名称**：*Development of a Diagnostic Assay Using Recombinant IMP-3 Protein for Pancreatic Cancer Detection*
**作者**：Yamamoto, M., et al.
**摘要**：利用重组IMP-3蛋白开发了一种新型ELISA检测法，证明其在胰腺癌患者血清中的高表达水平，提示其作为潜在液体活检标志物的临床价值。
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如需具体文献，建议通过PubMed或Web of Science以“recombinant IMP-3 protein”、“IMP-3 expression”等关键词检索近年研究。
Recombinant human IMP-3 (rhIMP-3) is a protein of interest in biomedical research due to its roles in RNA regulation and cancer biology. IMP-3. also known as insulin-like growth factor 2 mRNA-binding protein 3. belongs to the IGF2 mRNA-binding protein (IMPs/VICKZ) family, which governs RNA stability, localization, and translation during development and disease. While IMP-3 is essential for embryonic growth, its expression is typically low in healthy adult tissues. However, it is frequently overexpressed in malignancies such as hepatocellular, ovarian, and gastric cancers, correlating with tumor aggressiveness, metastasis, and poor prognosis. This oncofetal protein is implicated in promoting cancer cell proliferation, invasion, and therapy resistance by modulating oncogenic mRNA targets.
RhIMP-3 is produced via recombinant DNA technology, often expressed in bacterial or mammalian systems for functional studies. Its recombinant form enables in vitro exploration of molecular mechanisms underlying IMP-3’s pathological roles, including RNA-protein interactions, post-transcriptional gene regulation, and signaling pathways. Researchers also utilize rhIMP-3 to develop diagnostic tools, such as antibodies for detecting IMP-3 in cancer biopsies, and to screen potential inhibitors targeting its RNA-binding domains.
As a proposed biomarker and therapeutic target, rhIMP-3 remains central to understanding IMP-3’s dual roles in development and oncogenesis, bridging fundamental research with translational applications.
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