| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ILK-2 |
| Uniprot No | Q13418 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452aa |
| 氨基酸序列 | MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLTKLN ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV LPVLGACQSP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK |
| 分子量 | 51.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ILK-2(整合素连接激酶2)蛋白的模拟参考文献示例，供参考：
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1. **文献名称**：*Recombinant Human ILK-2 Expression and Functional Characterization in Cancer Cell Signaling*
**作者**：Zhang, L., et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组人ILK-2蛋白，验证其激酶活性，并证明其通过磷酸化Akt/PKB通路调控肿瘤细胞侵袭和迁移。
2. **文献名称**：*Structural Insights into ILK-2 Kinase Domain by X-ray Crystallography*
**作者**：Thompson, R.W., et al.
**摘要**：通过X射线晶体学解析了重组人ILK-2激酶结构域的3D结构，揭示了其与ATP结合口袋的关键位点，为靶向药物设计提供了结构基础。
3. **文献名称**：*ILK-2 Regulates Cardiac Fibrosis via TGF-β/Smad Pathway*
**作者**：Chen, H., et al.
**摘要**：利用哺乳动物细胞表达的重组ILK-2蛋白，发现其通过增强TGF-β/Smad信号传导促进心脏成纤维细胞活化，提示其与纤维化疾病的相关性。
4. **文献名称**：*Development of an ILK-2-Specific Inhibitor Using Recombinant Protein Screening*
**作者**：Saito, M., et al.
**摘要**：基于重组人ILK-2蛋白的高通量筛选平台，鉴定出新型小分子抑制剂，并验证其对炎症反应的抑制作用。
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**注**：以上参考文献为模拟内容，实际研究中ILK-2相关文献可能较少(因ILK家族以ILK-1为主)。建议进一步核实蛋白名称或扩展关键词检索(如“ILK isoform 2”)。
Recombinant human ILK-2 (Integrin-Linked Kinase 2) protein is a engineered variant of the ILK family, a group of intracellular serine/threonine kinases that interact with integrin β subunits to mediate cell-matrix adhesion and signaling. ILK-2. though less studied than its homolog ILK-1. plays roles in regulating cell proliferation, survival, and migration by influencing downstream pathways such as Akt/PKB and GSK-3β. It is implicated in cellular responses to mechanical stress, tissue remodeling, and cancer progression. Recombinant ILK-2 is typically produced using expression systems like E. coli or mammalian cells, enabling researchers to study its structure, enzymatic activity, and interactions in vitro. Its applications include elucidating mechanisms of fibrotic diseases, tumor metastasis, and cardiovascular disorders, as dysregulated ILK signaling is linked to pathological extracellular matrix deposition and epithelial-mesenchymal transition. The recombinant form also serves as a tool for drug screening targeting ILK-associated pathways. Despite its functional overlap with ILK-1. ILK-2 exhibits distinct regulatory features and substrate preferences, warranting focused investigation to clarify its unique biological contributions.
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