| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL1F7 |
| Uniprot No | Q9NZH6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-218aa |
| 氨基酸序列 | MSFVGENSGVKMGSEDWEKDEPQCCLEDPAGSPLEPGPSLPTMNFVHTSPKVKNLNPKKFSIHDQDHKVLVLDSGNLIAVPDKNYIRPEIFFALASSLSSASAEKGSPILLGVSKGEFCLYCDKDKGQSHPSLQLKKEKLMKLAAQKESARRPFIFYRAQVGSWNMLESAAHPGWFICTSCNCNEPVGVTDKFENRKHIEFSFQPVCKAEMSPSEVSD |
| 分子量 | 51.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IL1F7(IL-37)蛋白的3篇代表性文献及其摘要概述：
1. **"IL-37 is a fundamental inhibitor of innate immunity"**
- **作者**: Nold M.F. et al. (Dinarello C.A.课题组)
- **摘要**: 首次阐明IL-37(IL1F7)的抗炎功能，发现其通过抑制NF-κB通路和促炎细胞因子(如TNF-α、IL-6)的产生，在先天免疫中发挥关键调控作用，并在小鼠模型中证实重组IL-37可减轻炎症反应。
2. **"Crystal structure of IL-37 reveals the molecular basis for receptor binding and signaling"**
- **作者**: Wang X. et al.
- **摘要**: 解析了重组人IL-37蛋白的三维晶体结构，揭示了其与IL-18受体β链的结合机制，并阐明其抑制炎症的分子基础，为基于结构的药物设计提供了依据。
3. **"Recombinant IL-37 suppresses macrophage-mediated inflammation in vitro and prevents experimental colitis in vivo"**
- **作者**: McNamee E.N. et al.
- **摘要**: 证明重组IL-37蛋白可显著抑制巨噬细胞炎症反应，减轻小鼠结肠炎模型的肠道损伤，提示其在治疗炎症性肠病(IBD)中的潜在应用价值。
另附备选文献(如需第四篇)：
4. **"IL-37 requires the receptors IL-18Rα and IL-1R8 (SIGIRR) to carry out its multifaceted anti-inflammatory program"**
- **作者**: Ciccia F. et al.
- **摘要**: 揭示重组IL-37的抗炎作用依赖IL-18Rα和IL-1R8受体复合物，通过阻断下游信号传导降低炎症反应，为靶向治疗自身免疫疾病提供机制依据。
以上文献可从PubMed或期刊官网检索全文。如需具体发表年份或DOI，请进一步告知!
Interleukin-1 family member 7 (IL1F7), also known as IL-37. is an anti-inflammatory cytokine belonging to the IL-1 family. Discovered in 2000. it is encoded by the IL37 gene in humans and functions as a natural suppressor of innate immunity and inflammation. Unlike pro-inflammatory IL-1 family members (e.g., IL-1β, IL-18), IL-37 broadly dampens immune responses by inhibiting NF-κB and MAPK signaling pathways, reducing the production of pro-inflammatory cytokines (e.g., TNF-α, IL-6). It exists in multiple isoforms (IL-37a to IL-37e), with IL-37b being the most studied. Recombinant human IL1F7 protein is generated via genetic engineering, enabling studies on its structure-function relationships and therapeutic potential. It binds to IL-18 receptor α (IL-18Rα) and recruits the orphan receptor IL-1R8 (SIGIRR) to form a signaling complex, mediating its immunosuppressive effects. Research highlights its role in autoimmune diseases (e.g., rheumatoid arthritis, lupus), chronic inflammation, and sepsis, where excessive inflammation drives pathology. Preclinical models show that recombinant IL-37 alleviates tissue damage and improves outcomes by modulating immune cell activity (e.g., macrophages, T cells). Its clinical relevance is under investigation, particularly for conditions lacking targeted anti-inflammatory therapies. Production in systems like E. coli or mammalian cells ensures proper folding and post-translational modifications, preserving bioactivity for experimental and therapeutic applications.
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