Cat: PA2000-1733

Recombinant Human GPC Protein,His

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关键信息

  • 基因名

    GPC

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    GPC;Glypican-6

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P04921

  • 表达区间

    1-57aa

  • 氨基酸序列

    ADPMWSTRSPNSTAWPLSLEPDPGMASASTTMHTTTIAEPDPGMSGWPDG RMETSTPTIMHHHHHH

  • 分子量

    7 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

GPC (glypican) is a family of heparan sulfate proteoglycans that play pivotal roles in various cellular processes, including growth, differentiation, and regulation of cell signaling pathways. Research into GPC has gained momentum due to its involvement in pivotal biological functions and pathological conditions, such as cancer progression and development. GPCs are characterized by their attachment to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor, allowing them to interact with a range of ligands and modulate signaling pathways, such as those involving fibroblast growth factors (FGFs) and Wnt proteins. The role of GPCs in cancer biology is particularly notable, as they are often found to be upregulated in various tumor types, contributing to tumor growth, metastasis, and resistance to therapy. Thus, GPCs serve not only as potential biomarkers for cancer diagnosis and prognosis but also as promising therapeutic targets. Advances in recombinant protein technology have enabled the production and characterization of GPC proteins, facilitating deeper insights into their structure-function relationships and biological activities. Understanding the intricate mechanisms by which GPCs influence cellular processes is crucial for developing novel therapeutic strategies and improving patient outcomes in cancer treatment. As research continues to uncover the complexities of GPC interactions within the tumor microenvironment, the potential for innovative therapeutic interventions targeting GPCs is becoming increasingly viable. Overall, GPCs represent an exciting area of study in the realm of cell biology and oncology, with significant implications for both basic research and clinical applications.

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