| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IKBKE |
| Uniprot No | Q14164 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-716aa |
| 氨基酸序列 | MQSTANYLWH TDDLLGQGAT ASVYKARNKK SGELVAVKVF NTTSYLRPRE VQVREFEVLR KLNHQNIVKL FAVEETGGSR QKVLVMEYCS SGSLLSVLES PENAFGLPED EFLVVLRCVV AGMNHLRENG IVHRDIKPGN IMRLVGEEGQ SIYKLTDFGA ARELDDDEKF VSVYGTEEYL HPDMYERAVL RKPQQKAFGV TVDLWSIGVT LYHAATGSLP FIPFGGPRRN KEIMYRITTE KPAGAIAGAQ RRENGPLEWS YTLPITCQLS LGLQSQLVPI LANILEVEQA KCWGFDQFFA ETSDILQRVV VHVFSLSQAV LHHIYIHAHN TIAIFQEAVH KQTSVAPRHQ EYLFEGHLCV LEPSVSAQHI AHTTASSPLT LFSTAIPKGL AFRDPALDVP KFVPKVDLQA DYNTAKGVLG AGYQALRLAR ALLDGQELMF RGLHWVMEVL QATCRRTLEV ARTSLLYLSS SLGTERFSSV AGTPEIQELK AAAELRSRLR TLAEVLSRCS QNITETQESL SSLNRELVKS RDQVHEDRSI QQIQCCLDKM NFIYKQFKKS RMRPGLGYNE EQIHKLDKVN FSHLAKRLLQ VFQEECVQKY QASLVTHGKR MRVVHETRNH LRLVGCSVAA CNTEAQGVQE SLSKLLEELS HQLLQDRAKG AQASPPPIAP YPSPTRKDLL LHMQELCEGM KLLASDLLDN NRIIERLNRV PAPPDV |
| 分子量 | 80.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IKBKE蛋白的参考文献示例(请注意，这些为假设性示例，建议通过学术数据库检索真实文献)：
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1. **文献名称**：*"Expression and Functional Analysis of Recombinant Human IKBKE in Inflammatory Signaling"*
**作者**：Chen, L., et al.
**摘要**：研究报道了在昆虫细胞系统中高效表达重组人IKBKE蛋白，并通过体外激酶实验证实其对IRF3的磷酸化作用，揭示了IKBKE在调控I型干扰素通路中的关键功能。
2. **文献名称**：*"Structural Characterization of IKBKE Using Cryo-EM and Its Implications in Cancer Drug Design"*
**作者**：Park, S., et al.
**摘要**：通过冷冻电镜解析重组人IKBKE蛋白的构象变化，发现其与TAK1的相互作用界面，为设计选择性小分子抑制剂提供了结构基础。
3. **文献名称**：*"Development of a High-Throughput Screening Assay for IKBKE Kinase Activity Using Recombinant Protein"*
**作者**：Thompson, R., et al.
**摘要**：利用重组人IKBKE蛋白建立高通量激酶活性检测平台，筛选出多个潜在抑制剂，其中化合物X在乳腺癌细胞中显著抑制NF-κB信号通路。
4. **文献名称**：*"IKBKE Mutants Expressed in E. coli Reveal Autophosphorylation-Dependent Regulation"*
**作者**：Garcia, M., et al.
**摘要**：在大肠杆菌中表达并纯化重组人IKBKE突变体，证明其自磷酸化活性对先天免疫应答中的信号转导具有调控作用。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词如 *"recombinant IKBKE protein"*、*"IKBKE kinase assay"* 或 *"IKBKE structure function"* 获取真实文献。
Recombinant human IKBKE (Inhibitor of Nuclear Factor Kappa B Kinase subunit epsilon), also known as IKKε, is a serine/threonine kinase belonging to the IKK (IκB kinase) family. It plays a critical role in regulating NF-κB and interferon signaling pathways, which are central to innate immunity, inflammation, and cellular stress responses. Unlike canonical IKKs (IKKα and IKKβ), IKBKE is induced by viral infections or proinflammatory cytokines, such as TNF-α and IL-1β, and activates NF-κB indirectly by phosphorylating regulatory components like IRS-1 or STAT1. It also modulates IRF3/7-mediated type I interferon production, bridging antiviral defense and inflammatory cascades.
Dysregulation of IKBKE is linked to cancers (e.g., breast, ovarian, prostate) due to its oncogenic potential in promoting cell survival, proliferation, and metastasis. Recombinant IKBKE protein is engineered via heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its purified form enables biochemical assays, including kinase activity profiling, inhibitor screening, and interaction mapping with substrates or regulatory proteins. Researchers utilize recombinant IKBKE to dissect its role in disease mechanisms and explore therapeutic targeting, particularly in oncology and antiviral drug development. Structural analyses of recombinant IKBKE also aid in designing selective inhibitors to mitigate pathological signaling.
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