| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGSF10 |
| Uniprot No | Q6WRI0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 301-399aa |
| 氨基酸序列 | SAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQ |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IGSF10蛋白的虚构参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**: *Structural and Functional Characterization of Recombinant Human IGSF10 Protein*
**作者**: Liu, Y. et al.
**摘要**: 研究报道了通过哺乳动物表达系统成功表达并纯化重组人IGSF10蛋白，解析其晶体结构，并发现其通过调控Wnt信号通路参与细胞黏附和迁移。
2. **文献名称**: *IGSF10 Deficiency Disrupts Neuronal Migration in Mouse Models*
**作者**: Smith, J.R., & Tanaka, K.
**摘要**: 在小鼠模型中证实IGSF10蛋白对胚胎期神经元迁移至关重要，重组IGSF10的体外表达可部分挽救迁移缺陷，提示其治疗神经系统发育异常的潜力。
3. **文献名称**: *IGSF10 Mutations in Human Reproductive Disorders: Implications for Recombinant Protein Therapy*
**作者**: Chen, H. et al.
**摘要**: 通过对不孕症患者的基因测序发现IGSF10基因突变，实验表明重组人IGSF10蛋白可恢复体外培养生殖细胞的正常分化和功能。
4. **文献名称**: *Recombinant IGSF10 as a Biomarker for Early Cancer Detection*
**作者**: Gupta, S. & Wang, L.
**摘要**: 探索血清中重组IGSF10蛋白水平与多种癌症(如结直肠癌)的关联，提出其作为早期诊断生物标志物的可能性。
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**注**：以上为模拟示例，实际文献请通过PubMed、Google Scholar或Web of Science等平台以关键词"Recombinant human IGSF10"或"IGSF10 protein function"检索。
**Background of Recombinant Human IGSF10 Protein**
Immunoglobulin superfamily member 10 (IGSF10) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by extracellular immunoglobulin-like domains involved in cell adhesion and signaling. IGSF10 is evolutionarily conserved and plays critical roles in embryonic development, particularly in neuronal migration, tissue organization, and organogenesis. Studies highlight its importance in brain development, where it regulates the radial migration of neurons in the cerebral cortex. Dysregulation of IGSF10 has been linked to developmental disorders, including structural brain anomalies and reproductive system defects, as shown in animal models and human genetic analyses.
Recombinant human IGSF10 protein is produced using expression systems such as mammalian cells (e.g., HEK293 or CHO) to ensure proper post-translational modifications and functionality. It is purified via affinity chromatography, often with tags like His or Fc for enhanced stability. This recombinant form enables in vitro and in vivo studies to dissect IGSF10’s molecular mechanisms, including its interactions with extracellular matrix components, receptors, or signaling pathways (e.g., MAPK/ERK). Research applications span developmental biology, neurobiology, and reproductive health, with potential implications in understanding infertility, neurodevelopmental disorders, or cancer. However, the full scope of IGSF10’s physiological roles and therapeutic relevance remains under investigation, necessitating further structural and functional studies.
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