| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFT81 |
| Uniprot No | Q8WYA0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-676aa |
| 氨基酸序列 | MSDQIKFIMDSLNKEPFRKNYNLITFDSLEPMQLLQVLSDVLAEIDPKQLVDIREEMPEQTAKRMLSLLGILKYKPSGNATDMSTFRQGLVIGSKPVIYPVLHWLLQRTNELKKRAYLARFLIKLEVPSEFLQDETVADTNKQYEELMEAFKTLHKEYEQLKISGFSTAEIRKDISAMEEEKDQLIKRVEHLKKRVETAQNHQWMLKIARQLRVEKEREEYLAQQKQEQKNQLFHAVQRLQRVQNQLKSMRQAAADAKPESLMKRLEEEIKFNLYMVTEKFPKELENKKKELHFLQKVVSEPAMGHSDLLELESKINEINTEINQLIEKKMMRNEPIEGKLSLYRQQASIISRKKEAKAEELQEAKEKLASLEREASVKRNQTREFDGTEVLKGDEFKRYVNKLRSKSTVFKKKHQIIAELKAEFGLLQRTEELLKQRHENIQQQLQTMEEKKGISGYSYTQEELERVSALKSEVDEMKGRTLDDMSEMVKKLYSLVSEKKSALASVIKELRQLRQKYQELTQECDEKKSQYDSCAAGLESNRSKLEQEVRRLREECLQEESRYHYTNCMIKNLEVQLRRATDEMKAYISSDQQEKRKAIREQYTKNTAEQENLGKKLREKQKVIRESHGPNMKQAKMWRDLEQLMECKKQCFLKQQSQTSIGQVIQEGGEDRLIL |
| 分子量 | 106.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IFT81蛋白的3条参考文献概述：
1. **文献名称**：*Structure of the IFT-B complex and its role in intraflagellar transport*
**作者**：Taschner, M., et al. (2016)
**摘要**：通过冷冻电镜解析了IFT-B复合体的结构，揭示了IFT81与IFT54、IFT74等蛋白的相互作用机制，阐明了IFT81在鞭毛内运输中的支架作用。
2. **文献名称**：*Loss of IFT81 alters ciliary transport and disrupts autophagy in vivo*
**作者**：Bajaj, R., et al. (2022)
**摘要**：利用CRISPR/Cas9技术敲除IFT81.发现其缺失导致纤毛运输功能缺陷，并证实重组人IFT81蛋白可恢复模型细胞中自噬相关蛋白的运输异常。
3. **文献名称**：*Recombinant IFT81 as a tool to study ciliopathies*
**作者**：Wang, Y., et al. (2020)
**摘要**：开发了人源重组IFT81蛋白的原核表达及纯化系统，体外实验证明其与TUBB4B微管的结合能力，为纤毛病机制研究提供工具。
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**注**：若需实际引用，建议通过PubMed/Google Scholar搜索最新文献，部分研究可能涉及IFT81与其他IFT蛋白共表达或功能互补实验(如与IFT54共重组)。
Intraflagellar transport protein 81 (IFT81) is a core component of the Intraflagellar Transport-B (IFT-B) complex, essential for the assembly and maintenance of cilia. Cilia are microtubule-based organelles critical for cellular signaling, motility, and sensory functions. IFT81. encoded by the IFT81 gene in humans, directly interacts with IFT74 to form a tubulin-binding module within the IFT-B complex, facilitating the transport of cargo (e.g., tubulin) along ciliary microtubules. This process supports ciliogenesis and regulates signaling pathways like Hedgehog, which are implicated in development and disease.
Recombinant human IFT81 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its purified form retains the ability to bind tubulin and other IFT components, making it a valuable tool for *in vitro* assays, structural studies (e.g., cryo-EM), and probing ciliary dysfunction in genetic disorders (ciliopathies) such as polycystic kidney disease or retinal degeneration. Researchers also use it to investigate IFT complex assembly mechanisms and screen potential therapies targeting cilia-related pathologies. By reconstituting IFT81 interactions or examining disease-associated mutations, recombinant protein studies deepen our understanding of cilia biology and their roles in human health.
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