| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFT57 |
| Uniprot No | Q9NWB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-429aa |
| 氨基酸序列 | MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALKETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVMEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATVIPEPATGFY |
| 分子量 | 75.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IFT57蛋白的3条参考文献(模拟生成，非真实文献)：
1. **文献名称**：*IFT57 is essential for ciliary assembly and Hedgehog signaling in mammalian development*
**作者**：Tran PV, et al.
**摘要**：研究通过重组人IFT57蛋白体外表达，揭示了其在哺乳动物纤毛形成中的关键作用，并证明IFT57缺失导致纤毛结构异常，影响Hedgehog信号通路传导，与胚胎发育缺陷相关。
2. **文献名称**：*Functional analysis of recombinant IFT57 reveals its role in intraflagellar transport complex B assembly*
**作者**：Lucker BF, Cole DG.
**摘要**：通过重组表达和生化实验，证明IFT57蛋白是纤毛内运输复合体B(IFT-B)的核心组分，参与复合体的稳定性及与驱动蛋白Kinesin-II的结合，调控纤毛内运输过程。
3. **文献名称**：*Mutations in IFT57 disrupt clathrin-mediated endocytosis and cause skeletal ciliopathies*
**作者**：Wei Q, et al.
**摘要**：利用重组人IFT57进行功能回补实验，发现IFT57突变不仅破坏纤毛形成，还影响细胞内存作用相关蛋白(如网格蛋白)的定位，首次将IFT57异常与骨骼发育异常型纤毛病联系起来。
注：以上文献为示例性质，实际研究中建议通过数据库(如PubMed)检索具体论文。
**Background of Recombinant Human IFT57 Protein**
IFT57 (Intraflagellar Transport 57), also known as HIPPI, is a key component of the intraflagellar transport (IFT) complex B, which mediates cargo trafficking along the axoneme of cilia and flagella. Cilia are critical sensory and signaling organelles involved in developmental pathways (e.g., Hedgehog signaling) and cellular homeostasis. IFT57 interacts with other IFT-B proteins, such as IFT52 and IFT20. forming a core subcomplex essential for assembling and maintaining the IFT machinery. It plays a pivotal role in coordinating the movement of kinesin-driven IFT trains, ensuring proper ciliogenesis and function.
Defects in IFT57 are linked to ciliopathies, disorders characterized by abnormal ciliary structure or function, which may manifest as retinal degeneration, polycystic kidney disease, or skeletal abnormalities. Recombinant human IFT57 protein, typically produced in *E. coli* or mammalian expression systems with affinity tags (e.g., His or GST), enables *in vitro* studies of IFT complex assembly, protein-protein interactions, and ciliary transport mechanisms. It serves as a tool for investigating disease-related mutations, screening therapeutic agents, or developing targeted therapies for ciliopathies. Its structural and functional analysis further contributes to understanding broader roles in cellular signaling and organelle biogenesis.
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