| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFIT5 |
| Uniprot No | Q13325 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-482aa |
| 氨基酸序列 | MSEIRKDTLKAILLELECHFTWNLLKEDIDLFEVEDTIGQQLEFLTTKSRLALYNLLAYVKHLKGQNKDALECLEQAEEIIQQEHSDKEEVRSLVTWGNYAWVYYHMDQLEEAQKYTGKIGNVCKKLSSPSNYKLECPETDCEKGWALLKFGGKYYQKAKAAFEKALEVEPDNPEFNIGYAITVYRLDDSDREGSVKSFSLGPLRKAVTLNPDNSYIKVFLALKLQDVHAEAEGEKYIEEILDQISSQPYVLRYAAKFYRRKNSWNKALELLKKALEVTPTSSFLHHQMGLCYRAQMIQIKKATHNRPKGKDKLKVDELISSAIFHFKAAMERDSMFAFAYTDLANMYAEGGQYSNAEDIFRKALRLENITDDHKHQIHYHYGRFQEFHRKSENTAIHHYLEALKVKDRSPLRTKLTSALKKLSTKRLCHNALDVQSLSALGFVYKLEGEKRQAAEYYEKAQKIDPENAEFLTALCELRLSI |
| 分子量 | 78.76 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IFIT5蛋白的3篇示例文献摘要(注：文献为模拟内容，实际引用请核实原文)：
1. **文献名称**：*Expression and antiviral activity of recombinant human IFIT5 protein*
**作者**：Zhang Y, et al. (2020)
**摘要**：本研究在昆虫细胞系统中成功表达并纯化重组人IFIT5蛋白，证实其通过结合病毒RNA抑制多种RNA病毒(如丙型肝炎病毒)的复制，揭示了其在先天免疫中的潜在抗病毒机制。
2. **文献名称**：*Structural insights into the tetratricopeptide repeats of human IFIT5*
**作者**：Smith JL, et al. (2018)
**摘要**：通过大肠杆菌重组表达获得人IFIT5蛋白，利用X射线晶体学解析其三维结构，明确了其TPR结构域与RNA结合的关键位点，为靶向设计抗病毒药物提供结构基础。
3. **文献名称**：*IFIT5 regulates viral RNA recognition in innate immune signaling*
**作者**：Lee H, et al. (2019)
**摘要**：构建重组人IFIT5蛋白并通过体外实验证明其特异性识别5'-三磷酸RNA，干扰病毒基因组复制，研究进一步揭示了IFIT5在RIG-I通路中的调控作用。
(提示：实际研究中IFIT5重组蛋白的文献可能有限，建议结合具体数据库如PubMed或Web of Science检索最新成果。)
Human IFIT5 (Interferon-Induced Protein with Tetratricopeptide Repeats 5) is a key member of the IFIT family, a group of interferon-stimulated genes (ISGs) that play critical roles in innate antiviral immunity. Encoded by the IFIT5 gene, this protein is highly conserved and primarily induced by type I interferons (IFNs) in response to viral infections. Structurally, IFIT5 contains multiple tetratricopeptide repeat (TPR) motifs, enabling it to interact with viral RNA or other proteins to inhibit viral replication. It specifically targets 5’-triphosphate-containing RNA, a molecular signature of many RNA viruses, thereby blocking viral translation or recruiting immune effectors.
Recombinant human IFIT5 protein is produced using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity. Its production enables detailed studies on RNA-protein interactions, antiviral mechanisms, and immune signaling pathways. Researchers utilize recombinant IFIT5 to explore its role in restricting viruses (e.g., influenza, Zika, or coronaviruses), as well as its potential cross-talk with other immune sensors like RIG-I or MDA5. Additionally, IFIT5’s involvement in autoimmune diseases and cancer is under investigation, highlighting its dual role in immunity and pathology. The recombinant form provides a standardized tool for diagnostics, drug screening, and therapeutic development, bridging gaps in understanding host-virus dynamics and interferon-mediated defenses.
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