| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IER5 |
| Uniprot No | Q5VY09 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MEFKLEAHRIVSISLGKIYNSRVQRGGIKLHKNLLVSLVLRSARQVYLSDPCPGLYLAGPAGTPAPPPQQQPGEPAAGPPAGWGEPPPPAARASWPETEPQPERSSVSDAPRVGDEVPVATVTGVGDVFQGGEADATEAAWSRVEGPRQAAAREAEGTAGGWGVFPEVSRAARRPCGCPLGGEDPPGTPAATPRAACCCAPRPAEDEPPAPPAVCPRKRCAAGVGGGPAGCPAPGSTPLKKPRRNLEQPPSGGEDDDAEEMETGNVANLISIFGSSFSGLLRKSPGGGREEEEGEESGPEAAEPGQICCDKPVLRDMNPWSTAIVAF |
| 分子量 | 60.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IER5蛋白的3篇示例文献(注：以下文献为模拟虚构，实际文献需通过数据库查询确认)：
1. **文献名称**：*"Recombinant Human IER5 Promotes Apoptosis in Cancer Cells via Interaction with Hsp90"*
**作者**：Zhang L. et al.
**摘要**：研究发现重组人IER5蛋白通过与热休克蛋白Hsp90结合，抑制其功能，导致肿瘤细胞内促凋亡蛋白的激活，进而诱导癌细胞凋亡，提示其在癌症治疗中的潜在应用价值。
2. **文献名称**：*"IER5 Regulates DNA Damage Repair Through Modulating ATM Kinase Activity"*
**作者**：Chen Y. et al.
**摘要**：该文献揭示了重组人IER5蛋白在电离辐射诱导的DNA损伤反应中的作用，发现其通过增强ATM激酶活性，促进下游修复信号通路激活，帮助维持基因组稳定性。
3. **文献名称**：*"Role of Recombinant IER5 in Cell Cycle Checkpoint Control via PP2A Interaction"*
**作者**：Wang X. et al.
**摘要**：研究表明，重组人IER5蛋白通过与蛋白磷酸酶PP2A的相互作用，调控细胞周期检查点，影响G2/M期转换，为理解其在细胞应激反应中的功能提供了机制基础。
如需查找真实文献，建议使用PubMed或Web of Science等平台，关键词：**Recombinant Human IER5**, **IER5 function**, **DNA damage response**, **Cancer therapy**。
Recombinant human IER5 (Immediate Early Response 5) protein is a genetically engineered version of the naturally occurring IER5 protein, which belongs to the immediate early gene family. These genes are rapidly activated in response to cellular stress signals, such as DNA damage, oxidative stress, or growth factor stimulation. The IER5 protein is implicated in regulating cellular processes like proliferation, apoptosis, and DNA repair. It interacts with protein phosphatase 2A (PP2A), modulating its activity to influence stress-responsive pathways, including the p53-mediated apoptosis and cell cycle arrest. Studies suggest its involvement in cancer biology, as dysregulated IER5 expression correlates with tumor progression and chemotherapy resistance.
Recombinant IER5 is typically produced using bacterial or mammalian expression systems, ensuring high purity and bioactivity for experimental applications. It serves as a critical tool for investigating IER5's molecular mechanisms, such as its role in heat shock response, genomic stability maintenance, and epigenetic regulation. Researchers utilize it in vitro and in vivo to study protein-protein interactions, gene expression profiling, and therapeutic targeting. Its recombinant form overcomes limitations of low endogenous protein levels, enabling standardized analysis across studies. Ongoing research aims to elucidate IER5’s dual roles in promoting cell survival under stress and suppressing oncogenesis, highlighting its potential as a biomarker or therapeutic target in diseases like cancer.
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